Description:
Quick-Load® 2-Log DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye.
The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference bands.
Effective Size Range: 100 bp to 10 kb
Source:
The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
Concentration:
100 μg/ml
Recommended Load: 1 μg
Storage Conditions:
3.3 mM Tris-HCl
11 mM EDTA
0.015% Bromophenol Blue
0.017% SDS
2.5% Ficoll 400
pH 8.0 @ 25°C
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