phi29 DNA Polymerase Reaction Buffer

产品编号：B0269S 品牌：New England Biolabs 原厂货号：B0269S
产品分类：生化试剂 > 生物缓冲液 > 分子生物酶缓冲液
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包装：

运保温度:

-20°C

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描述：

Description:
10X phi29 DNA Polymerase Reaction Buffer for use with phi29 DNA Polymerase and supplied as a 10X concentrated stock solution.

Reagents Supplied:
10X phi29 DNA Polymerase Reaction Buffer
BSA (10 mg/ml)

1X phi29 DNA Polymerase Reaction Buffer:
50 mM Tris-HCl
10 mM MgCl₂
10 mM (NH₄)₂SO₄
4 mM Dithiothreitol
pH 7.5 @ 25°C

Quality Control for Current Lot

16-Hour Incubation:
A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested ΦX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation.

Endonuclease Activity:
Incubation of this reaction buffer at a 1X concentration with 1 µg ΦX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II.