Taq 5X Master Mix

产品编号：M0285S 品牌：New England Biolabs 原厂货号：M0285S
产品分类：PCR/RT-PCR/qRT-PCR > PCR > 常规PCR
应用分类：

包装：

100 rxns (50 μl vol)

运保温度:

-20°C

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描述：

Description:
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).
Taq 5X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl₂, KCl and stabilizers. It is best for routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.

Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticusYT-1.

Applications:

PCR
Primer Extension
Colony PCR

Reagents Supplied:
MgCl₂ (25 mM)
Heat Inactivation:
No
Unit Definition:
One unit of Taq DNA Polymerase is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

Unit Assay Conditions:
1X ThermoPol^® Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 200 µg/ml activated Calf Thymus DNA.

Reaction Conditions:
1X Taq Master Mix, DNA template and primers in a total reaction volume of 25 or 50 µl.

1X Taq Master Mix:
10 mM Tris-HCl (pH 8.6 @ 25°C)
50 mM KCl
1.5 mM MgCl₂
25 units/ml Taq DNA Polymerase
0.2 mM dNTPs each
5% glycerol
0.08% IGEPAL^® CA-630
0.05% Tween-20

Concentration:
5 X