PURExpress Disulfide Bond Enhancer

• 产品编号：E6820S      品牌：New England Biolabs       原厂货号：E6820S
• 产品分类：蛋白质表达与纯化 > 蛋白质表达系统 > 无细胞表达系统
• 应用分类：

描述：

Description:
The PURExpress® Disulfide Bond Enhancer (PDBE) is a proprietary blend of proteins and buffer components designed to correctly fold target proteins with multiple disulfide bonds produced in PURExpress reactions. Added at the beginning of a reaction, the components promote a proper disulfide bond pattern by assisting with the oxidation of cysteine thiols and correcting mis-oxidized substrates. These enhancements can increase the yield of soluble and functionally active protein.

Reagents Supplied:
PURExpress Disulfide Bond Enhancer 1
PURExpress Disulfide Bond Enhancer 2

Reaction Conditions:
Add 1 µl of each component to a PURExpress reaction (as part of the user-added 7.5 µl volume for templates and additives) and incubate reaction at 37°C for at least 2 hours.
 

Applications:

Formation of properly folded proteins containing multiple disulfide bonds when produced by PURExpress.

Incubate at 37°C.

Storage Conditions:
50 mM HEPES
100 mM KCl
1 mM EDTA
10% Glycerol

原厂资料：