OneTaq GC Reaction Buffer Pack

• 产品编号：B9023S      品牌：New England Biolabs       原厂货号：B9023S
• 产品分类：生化试剂 > 生物缓冲液 > 分子生物酶缓冲液
• 应用分类：

描述：

Description:
5X OneTaq GC Reaction Buffer and OneTaq High GC Enhancer are optimized for use with OneTaq and OneTaq Hot Start DNA Polymerases. This reaction buffer and supplemental enhancer have been formulated for robust yields of difficult or GC-rich amplicons. The OneTaq GC Reaction Buffer is recommended for GC-rich (>65% GC) amplicons. For particularly difficult amplicons, the OneTaq High GC Enhancer can be added at a final concentration of 10–20% to reactions with OneTaq GC Reaction Buffer.

Reaction Conditions:
1X OneTaq GC Reaction Buffer, 10–20% OneTaq High GC Enhancer (optional), DNA template, primers, 200 µM dNTPs (not included) and 1.25 units of OneTaq or OneTaq Hot Start DNA Polymerase (not included) in a total reaction volume of 50 µl.
 

Reagents Supplied:
5X OneTaq GC Reaction Buffer
OneTaq High GC Enhancer

1X OneTaq GC Reaction Buffer:
80 mM Tris-SO₄
2 mM MgSO₄
20 mM (NH₄)₂SO₄
5 % Glycerol
5 % DMSO
0.06 % IGEPAL® CA-630
0.05 % Tween-20
pH 9.2 @ 25°C

OneTaq High GC Enhancer:
10 mM Tris-HCl
25% DMSO
25% Glycerol
pH 9.2 @ 25°C
 

原厂资料：