Description: 5X OneTaq® Standard Reaction Buffer is optimized for use with OneTaq and OneTaq Hot Start DNA Polymerases. This reaction buffer has been formulated for robust yields with minimal optimization. The OneTaq Standard Reaction Buffer is recommended for most routine and/or AT-rich amplicons (i.e. Lambda, etc.) or complex amplicons with up to ~65% GC content.
Reaction Conditions:
1X OneTaq Standard Reaction Buffer, DNA template, primers, 200 µM dNTPs (not included) and 1.25 units of OneTaq or OneTaq Hot Start DNA Polymerase (not included) in a total reaction volume of 50 µl.
Reagents Supplied: 5X OneTaq Standard Reaction Buffer
1X OneTaq Standard Reaction Buffer:
20 mM Tris-HCl
1.8 mM MgCl2
22 mM NH4Cl
22 mM KCl
0.06 % IGEPAL® CA-630
0.05 % Tween-20 pH 8.9 @ 25°C