Mouse VEGF R1/Flt-1 Quantikine ELISA Kit (1 KT)

产品编号：RD-MVR100 品牌：R&D 原厂货号：MVR100
产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
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描述：

Molecule Information: VEGF R1/Flt-1

Long Name: Vascular Endothelial Growth Factor Receptor 1

Aliases: FLT1; Flt-1

Entrez Gene IDs: 2321 (Human); 14254 (Mouse)

Background: VEGF R1, R3
VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

原厂资料：

Product Summary:

The Quantikine Mouse soluble VEGF R1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse sVEGF R1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse VEGF R1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse sVEGF R1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse sVEGF R1.

Precision:

Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, EDTA Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	265	1,383	2,174	250	1,339	2,139
Standard Deviation	19	55	69	21	96	134
CV%	7.2	4	3.2	8.4	7.2	6.3

Recovery:

The recovery of mouse sVEGF R1 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Serum (n=6)	106	96-119
Cell Culture Supernates (n=6)	96	82-110
EDTA Plasma (n=6)	106	92-120

Linearity:

To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of mouse sVEGF R1 in each matrix were diluted with Calibrator Diluent and then assayed.