Background:IL-1ra/IL-1F3
IL-1 receptor antagonist (IL-1ra) was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells. In mouse, IL-1RII does not bind IL-1ra.
原厂资料：
Product Summary:
The Quantikine Mouse RBP4 immunoassay is a 4.5 hour solid-phase ELISA designed to measure RBP4 in cell culture supernates, cell lysates, mouse serum, plasma, and urine. It contains NS0-expressed recombinant mouse RBP4 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant mouse RBP4. Results obtained using natural mouse RBP4 showed dose response curves that were parallel to the standard curve obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse RBP4.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in assays to assess inter-assay precision.
The recovery of mouse RBP4 spiked to three levels throughout the range of the assay was evaluated.
Sample Type
Average % Recovery
Range %
Cell Culture Supernates (n=4)
98
90-112
Urine (n=4)
91
80-107
Linearity:
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse RBP4 in each matrix were diluted with Calibrator Diluent and assayed.
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