Mouse/Rat CCL5/RANTES Quantikine ELISA Kit, 2 Plate (1 KT)

产品编号：RD-MMR00 品牌：R&D 原厂货号：MMR00
产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
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描述：

Molecule Information: CCL5/RANTES

Aliases: RANTES; SISd

Entrez Gene IDs: 6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)

Background: CCL5/RANTES
RANTES, also known as CCL5 and SISd, is a chemokine that belongs to the CC chemokine subfamily. It is a secreted polypeptide produced by a wide variety of epithelial and mesodermal cell types and is involved in both cellular and humoral immunity.

原厂资料：

Product Summary:

The Quantikine Mouse/Rat CCL5/RANTES Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat RANTES levels in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant mouse RANTES as well as antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant RANTES. Results obtained using natural mouse or rat RANTES showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse and rat RANTES.

Precision:

Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Citrate Plasma, Platelet-poor Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	18.8	104	198	20.1	109	208
Standard Deviation	0.7	1.9	4.1	1.6	5.6	11.5
CV%	3.7	1.8	2.1	8	5.1	5.5

Recovery:

The recovery of RANTES spiked to three levels in mouse samples throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Serum (n=5)	97	90-107
Cell Culture Supernates (n=8)	108	98-115
Platelet-poor Citrate Plasma (n=5)	102	95-110
Platelet-poor EDTA Plasma (n=5)	99	86-110

Linearity:

To assess the linearity of the assay, four or more samples containing or spiked with various concentrations of RANTES in each matrix were diluted with Calibrator Diluent and then assayed.