Mouse IGFBP-3 Quantikine ELISA Kit (1 KT)

产品编号：RD-MGB300 品牌：R&D 原厂货号：MGB300
产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
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描述：

Molecule Information: IGFBP-3

Long Name:Insulin-like Growth Factor Binding Protein 3

Entrez Gene IDs: 3486 (Human); 16009 (Mouse)

Background: IGFBP Family

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and several low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

原厂资料：

Product Summary:

The Quantikine Mouse IGFBP-3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IGFBP-3 in cell culture supernates and plasma. It contains NS0-expressed recombinant mouse IGFBP-3 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IGFBP-3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse IGFBP-3.

Precision:

Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	280	628	2,653	283	602	2,648
Standard Deviation	13.5	25.1	148	23.4	44.4	156
CV%	4.8	4	5.6	8.3	7.4	5.9

Recovery:

The recovery of mouse IGFBP-3 spiked to three levels throughout the range of the assay in the cell culture supernate samples was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Samples (n=4)	100	90-109

Linearity:

To assess the linearity of the assay, samples containing high concentrations of mouse IGFBP-3 in each matrix were diluted with Calibrator Diluent and assayed.