Description: Granzyme B, (CGL1,cathepsin G-like-1), is a member of the granzyme serine protease family found in the granules of cytotoxic T cells after activation and NK cells. Granzyme B is one of eleven granzymes found in the mouse genome and synthesized as a precursor of 247 amino acid residues. It consists of a signal sequence, a pro peptide and a mature chain. The amino acid sequence of mouse Granzyme B is 69% and 80% identical to human and rat Granzyme B.The name 'granzymes' is derived from: granules + enzymes. These granules contain next to granzymes other proteins including a poreforming protein (Perforin). Upon binding of the CTL to a target cell (by CTL-receptor and antigen-presenting MHC molecules on the target cell) the contents of the granules are released in the intercellular space where after perforine will perforate the target cell membrane by forming transmembrane pores. Through these pores the granzymes can now enter the cytosol of the target cell. Granzyme B activates the intracellular cascade of caspases finally resulting in the killing of the target cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved has been identified as mannose 6-phosphate receptor. This receptor functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis.
Measurement of release of Granzyme B in response to the appropriate target is useful for evaluating cell-mediated cytotoxicity. Granzyme B may have roles in rheumatoid arthritis and in bacterial and viral infection
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse Granzyme B
Biotin-Conjugate anti-mouse Granzyme B antibody
Streptavidin-HRP
Mouse Granzyme B Standard lyophilized, 1000 pg/ml upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Sample Diluent
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films