Description: The human sCD153 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD153. The human sCD153 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
The human CD30 ligand, CD153, is a 40 kDa Type II transmembrane glycoprotein. It interacts with its receptor, CD30, as a homotrimer. Soluble trimeric CD153 binds to membrane-anchored CD30 with a relatively high affinity and is very effective in triggering cell death and TNF-alpha . CD153 is a member of the TNF superfamily which is primarily expressed by activated T cells, B cells and monocytes. Its function in programmed cell death has been proven, as well as its involvement in disorders like a variety of lymphoid malignancies like Hodgkin?s disease and anaplastic large cell lymphoma.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD153
Biotin-Conjugate anti-human sCD153 monoclonal antibody
Streptavidin-HRP
Human sCD153 Standard lyophilized, 200 ng/ml upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
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