十二烷基硫酸钠,超纯(SDS)Sodium Dodecyl Sulfate, Ultra Pure

产品编号：04811034 品牌：MP 原厂货号：04811034
产品分类：生化试剂 > 化学试剂 > 表面活性剂（增溶剂和去污剂） > SDS
应用分类：蛋白质研究 > 蛋白质凝胶电泳
　　　　　暂定分类 > 膜蛋白表达 > SDS-PAGE > SDS-PAGE Buffers & Reagents

包装：

运保温度:

Room Temperature

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描述：

SDS; Lauryl sulfate sodium salt; Dodecyl sulfate sodium salt; Dodecyl sodium sulfate; Sodium lauryl sulfate; Sulfuric acid monododecyl ester sodium salt
Purity: >99%
OD280 (1% aq soln): <0.1
Heavy Metals (as Pb): <0.002%
Anionic detergent used to solubilize proteins.

Description: An anionic detergent³ typically used to solubilize⁸ and denature proteins for electrophoresis.^4,5 SDS has also been used in large-scale phenol extraction of RNA to promote the dissociation of protein from nucleic acids when extracting from biological material.¹² Most proteins bind SDS in a ratio of 1.4 grams SDS to 1 gram protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on protein size.

Typical Working Concentration: > 10 mg SDS/mg protein

Typical Buffer Compositions:

SDS Electrophoresis Gel Running Buffer

Component	Composition (g/L)	Molarity or %
Tris	3.035	0.025 M
Glycine	14.1	0.192 M
SDS	1	0.1%
pH 8.3

SDS Electrophoresis Gel Sample Solubilization Buffer

Component	Composition (g/L)	Molarity or %
Tris	15.16	0.125 M
SDS	20.0	2.0%
Sucrose	100	10.0%
Bromophenol Blue	0.2	0.02%

Titrated to pH 8.0 with HCl

十二烷基硫酸钠
用途：SDS是一种已知的能够使蛋白质变性的去污剂。它用于确定蛋白分子量的聚丙烯酰胺凝胶电泳。它也可以用于核酸抽提操作中破坏细胞壁及裂解核酸：蛋白复合物。在较高温度下，破坏蛋白质与DNA的结合，使DNA释放出来。在乳液聚合反应中，可充当两相溶液的乳化剂。