RAR A COACTIVATOR ASSAY KIT 800 X 20 UL ASSAYS

产品编号：PV4409 品牌：invitrogen 原厂货号：PV4409
产品分类：生化和细胞分析 > 生化分析试剂盒 > 核受体辅因子相互作用分析
应用分类：行业及应用 > 药物研发 > 靶标和先导药物鉴定及验证 > G 蛋白偶联受体生物学G-Protein Coupled Receptor Biology (GPCR) > Membrane Protein Expression System > 生物化学核受体分析 > 核受体分析 > LanthaScreen™ TR-FRET NR 辅调节因子相互作用分析

包装：

运保温度:

Store at -80°C

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描述：

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15122:

The LanthaScreen® TR-FRET Retinoic Acid Receptor (RAR) alpha Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential RAR alpha ligands as agonists of coactivator recruitment. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

How it works
The LanthaScreen® TR-FRET RAR alpha Coactivator Assay Kit includes a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human RAR alpha ligand-binding domain (RAR alpha-LBD) that is tagged with glutathione-S-transferase (GST). To assay, RAR alpha-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti-GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound.

Contents and Storage:
The LanthaScreen® TR-FRET RAR alpha Coactivator Assay Kit contains RAR alpha-LBD (GST) protein, fluorescein-labeled D22 coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

注意事项：

For Research Use Only. Not for use in diagnostic procedures.