MegaX DH10B™ T1R Electrocomp™ Cells are the highest-efficiency electrocompetent cells available. Improved production processes now guarantee a three-fold greater number of colonies per transformation (>3 x 1010 cfu/µg of pUC DNA), making the MegaX DH10B™ T1R cells ideal for highly demanding cloning and library construction. They have the same genotype as the widely used DH10B™ T1R strain including tonA, which prevents infection by T1 and T5 lytic bacteriophages1 and safeguards your valuable clones and libraries.
MegaX DH10B™ T1R cells provide highly efficient transformation of methylated DNA, since they lack the E. coli K12 restriction systems (mcrA Δ(mrr-hsdRMS-mcrBC)).2,3,4 The 80lacZΔM15 marker provides α-complementation of the β-galactosidase gene, allowing blue/white screening on agar plates containing X-gal or Bluo-gal.
Contents and Storage:
MegaX DH10B™ T1R Electrocomp™Cells, pUC19 DNA (10 pg/µl), and Recovery Medium are shipped on dry ice. Store at -80°C upon receipt.