5-amino-2,3-dihydro-1,4-phthalazinedione鲁米诺

产品编号：0219503805 品牌：MP 原厂货号：195038
产品分类：生化试剂 > 染料/染色剂/指示剂/底物 > 标记和检测产品 > 其它荧光染料
应用分类：蛋白质研究 > 免疫印迹 > 免疫印迹检测
　　　　　行业及应用 > 身份鉴定 > Validation

包装：

5 g

运保温度:

Room Temperature

到货周期：

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描述：

Luminol

Description: 5-Amino-2,3-dihydro-1,4-phthalazinedione is readily oxidized in basic solution, with the release of energy as visible light. The reaction can be carried out in a variety of media including protic solvents such as water, and aprotic solvents (DMSO or DMF). The mechanism of oxidation varies with the solvent, and slightly different conditions are needed. In aprotic media, only molecular oxygen and a strong base are needed to produce chemiluminescence (l_max = 485 nm). In aqueous systems, a strong base, either molecular oxygen or a peroxide and an auxiliary oxidant such as hypochlorite or perborate are required for chemiluminescence (l_max = 425 nm. The chemiluminescence spectrum of 5-Amino-2,3-dihydro-1,4-phthalazinedione indicates greatest relative intensity at 425 nm⁹). The actual form that emits light is the aminophthalate ion.⁹

Typical applications include microestimation of glucose and glucose oxidase using enzyme-induced chemiluminescence^3,7, inhibitor of poly ADP Ribose Synthase², and demonstrations of the phenomenon of chemiluminescence using different sensitizers to produce different colors of light.¹⁰
Typical Presumptive test for blood^{5, 13}: 3.5 g of Sodium perborate is added to 500 ml distilled water and thoroughly dissolved. 25 g of sodium carbonate and 9.5 g 5-Amino-2,3- dihydro- 1,4- phthalazinedione are then added and dissolved. The solution is allowed to stand for five minutes to allow any undissolved chemicals to settle. The solution is then decanted into a plastic spray bottle and is ready to use. It should be applied as a fine mist on the surface to be tested. Bloodstains will luminesce with an even glow that will last for several seconds (for best results, the area should be as dark as possible).
This is not a definitive test for blood since it is the iron in the heme which catalyzes the oxidation and the subsequent chemiluminescence. The presence of copper as a contaminant will accelerate the oxidation process.
Solutions can be tested by spraying on a clean piece of copper such as a penny.

鲁米诺；3-氨基苯二甲酰肼；发光氨

用途：化学发光试剂，常用于化学发光免疫分析，例如，金属阳离子和血液分析。
原理：血液中含有血红蛋白，血红蛋白含有铁，而铁能催化过氧化氢的分解，让过氧化氢变成水和单氧，单氧能氧化鲁米诺让它发光。在检验血痕时，鲁米诺与血红素（血红蛋白中负责运输氧的一种蛋白质）发生反应，显出蓝绿色的荧光。