吐温 80Tween 80

• 产品编号：02194725.1      品牌：MP       原厂货号：02194725.1
• 产品分类：生化试剂 > 化学试剂 > 表面活性剂（增溶剂和去污剂） > 吐温tween
• 应用分类：行业及应用 > 药物研发 > 药物研发服务

描述：

Cell Culture Reagent
Syrup
Purity: ∼75% oleic acid
Synonyms: Polyoxyethylenesorbitan monooleate

Structure (Tween® 80):¹⁹



Description: Tweens® are a series of nonionic surfactants derived from sorbitan esters. They are soluble or dispersible in water but differ widely in organic and oil solubilities. Used as oil-in-water emulsifiers in pharmaceuticals, cosmetics, cleaning compounds, etc. (Not sold by MP for human use).
 

	Polysorbate 20	Tween® 40	Tween® 60	Tween® 80	Tween® 85
CAS #	9005-64-5	9005-66-7	9005-67-8	9005-65-6	9005-70-3
Synonym	Polyoxyethylene- sorbitan monolaurate; Polysorbate 20	Polyoxyethylene- sorbitan monopalmitate; Polysorbate 40	Polyoxyethylene- sorbitan monostearate; Polysorbate 60	Polyoxyethylene- sorbitan monooleate; Polysorbate 80	Polyoxyethylene- sorbitan trioleate; Polysorbate 85
Content	~55% lauric acid (remainder primarily myristic, palmitic and stearic acid)	~90% palmitic acid	~55% stearic acid (remainder primarily palmitic acid)	~75% oleic acid	~70% oleic acid (remainder primarily elaidic, linolenic and palmitic acids)
Specific Gravity	~1.1 g/ml	~1.08 g/ml	~1.1 g/ml	~1.08 g/ml	~1.0 g/ml
Viscosity @ 25°C	400 cps	600 cps	--	400 cps	300 cps
Acid No.	0-2.0	0-2.0	0-2.0	0-2.0	0-2.0
Saponification No.	40-50	43-49	45-55	45-55	83-93
Hydroxyl No.	96-108	89-105	81-96	65-80	39-52
Water	2.5-3.0%	2.5-3.0%	2.5-3.0%	2.5-3.0%	4.8-5.2%
Critical Micelle Concen. (CMC)	0.06 mM (60 mg/L or  6 × 10-3 mole/L)	--	--	0.012 mM (13-15 mg/L)	--
Molecular Weight	1228	--	1311.7	1310	--

Physical Description: Yellow, oily liquid

Typical Usage:

Polysorbate 20 is typically used as an emulsifying agent for the preparation of stable oil-in-water emulsions, particularly in pharmaceutical applications.¹⁷ Polysorbate 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).^1,10,12 Several resources may be helpful in determining usage concentrations.^8,9,14Polysorbate 20 has been used as a blocking agent on nitrocellulose at a typical concentration of 0.05%.

Tween® 40 can be used as a non-ionic detergent in cell lysis, nuclei isolation and cell fractionation.^2,13,15

Tween® 80 has been widely used in biochemical applications including: solubilizing proteins, isolating nuclei from cells in culture^4,7,15, selective protein extraction^4,15, growing of tubercule bacilli⁶, and emulsifying and dispersing substances in medicinal and food products. It has little or no activity as an anti-bacterial agent.³ It has been shown to have an adverse effect on the antibacterial effect of methyl paraben and related compounds.¹⁶

Critical Micelle Concentration (CMC): Detergents with high CMC values are generally easy to remove by dilution; detergents with low CMC values are advantageous for separations on the basis of molecular weight. As a general rule, detergents should be used at their CMC and at a detergent-to-protein weight ratio of approximately ten. ^19-20

Solubility: Soluble/miscible in water to give a clear yellow solution; miscible with alcohol, dioxane, and ethyl acetate; practically insoluble in liquid paraffin and fixed oils (such as mineral oil).¹⁷ Autoclaving of solutions is not recommended. Sterile filtering is suggested with a 0.22 micron filter. Tween® may need to be warmed to about 40°C and alternated with portions of hot distilled water while being poured through the filter.

Availability:

功用作用： 吐温-20为月桂酯；吐温-60为硬脂酸酯；吐温-80为油酸酯，均有乳化作用。

　　可作混悬剂的润湿剂。非离子型表面活性剂 。

　　用法用量： 供配制水包油乳剂或乳膏时，用作乳化剂。也可用于油类的助溶（如挥发油）。

　　注意事项： （1）有溶血作用，以吐温-80作用最弱。

（3）在溶液中可干扰抑菌剂的作用

原厂资料：