描述:
Human Relaxin-3 (H3 relaxin, INSL7) is one of seven relaxin-like peptides belonging to the
insulin superfamily. Unlike human relaxins 1 and 2, it does not play a role in reproduction but
appears to be a neuropeptide involved in stress response in the brain stem. The 142 amino
acid (aa) Relaxin-3 preproprotein is processed to remove a 25 aa signal peptide and a
connecting peptide (aa 53-118). The resulting mature Relaxin-3 is a 5.5 kDa, 51 aa secreted
heterodimer of A (aa 119 -142) and B (aa 26 -52) peptides connected by two intermolecular
disulfide bonds. Mature human Relaxin-3 is 96%, 94%, and 92% aa identical to porcine, canine,
and mouse Relaxin-3, respectively. This is much higher identity between species than that seen
for other relaxins. Relaxin-3 is thus suggested to be the ancestral relaxin family member.
Relaxin-3 is the only known ligand for the G-protein-coupled receptor GPCR135, designated
RXFP3. In rodents, GPCR135 is expressed primarily in the supraoptic and paraventricular
nucleus. This region has connections to the dorsal tegmental region of the pons (also called
the nucleus incertus), where expression of Relaxin-3 is highest. Relaxin-3 also binds the more
widelyexpressed LGR7 (RXFP1) receptor, but with lower affinity than that of Relaxin-2.
Although binding of Relaxin-3 to LGR7 increases intracellular cAMP, binding to GPCR135 inhibits
cAMP accumulation, indicating coupling to Gi, Go or Gzby this receptor. Relaxin-3 expression
does not overlap well with its other receptor, GPCR142, which instead appears to be the
primary receptor for INSL5.
原厂资料:
Human Relaxin-3 (H3 relaxin, INSL7) is one of seven relaxin-like peptides belonging to the
insulin superfamily. Unlike human relaxins 1 and 2, it does not play a role in reproduction but
appears to be a neuropeptide involved in stress response in the brain stem. The 142 amino
acid (aa) Relaxin-3 preproprotein is processed to remove a 25 aa signal peptide and a
connecting peptide (aa 53-118). The resulting mature Relaxin-3 is a 5.5 kDa, 51 aa secreted
heterodimer of A (aa 119 -142) and B (aa 26 -52) peptides connected by two intermolecular
disulfide bonds. Mature human Relaxin-3 is 96%, 94%, and 92% aa identical to porcine, canine,
and mouse Relaxin-3, respectively. This is much higher identity between species than that seen
for other relaxins. Relaxin-3 is thus suggested to be the ancestral relaxin family member.
Relaxin-3 is the only known ligand for the G-protein-coupled receptor GPCR135, designated
RXFP3. In rodents, GPCR135 is expressed primarily in the supraoptic and paraventricular
nucleus. This region has connections to the dorsal tegmental region of the pons (also called
the nucleus incertus), where expression of Relaxin-3 is highest. Relaxin-3 also binds the more
widelyexpressed LGR7 (RXFP1) receptor, but with lower affinity than that of Relaxin-2.
Although binding of Relaxin-3 to LGR7 increases intracellular cAMP, binding to GPCR135 inhibits
cAMP accumulation, indicating coupling to Gi, Go or Gzby this receptor. Relaxin-3 expression
does not overlap well with its other receptor, GPCR142, which instead appears to be the
primary receptor for INSL5.