HYDROCORTISONE

• 产品编号：0219456901      品牌：MP       原厂货号：194569
• 产品分类：生化试剂 > 生化试剂 > 常用化学试剂
• 应用分类：

描述：

Hydrocortisone

Cell Culture Reagent
Sulfuric acid solutions fluoresce green

Catalog Number: 101996, 194568, 194569, 199339

Structrure:

Synonyms: Cortisol; 17-Hydroxycorticosterone; Kendall's Compound F; 4-Pregnene-11b,17a,21-triol-3,20-dione; Reichstein's Substance M; 11b,17a,21-Trihydroxypregn-4-ene-3,20-dione

Physical Description: White crystalline powder

Source: Soy beans

Solubility: Soluble (mg/ml) at 25^oC in: water 0.28; ethanol 15.0; methanol 6.2; acetone 9.3; chloroform 1.6; propylene glycol 12.7; ether ~ 0.35. Soluble in concentrated sulfuric acid with intense green fluorescence.¹ 

For the water soluble hydrocortisone (cat#: 199339): Typical stock solutions can be prepared at concentrations of 50-500 mg solid (total powder) in 1 ml water (difficulty may be encountered when attempting to solubilize in salt solutions or buffers). Solutions can be sterile filtered using a 0.2 um membrane. Solutions should be stored in working aliquots at -20°C. When calculating molar solutions, the molecular weight of the cyclodextrin component of the complex is typically disregarded and the solution is prepared considering the amount of the hydrocortisone in the preparation (100 mg/gram solid) and its respective molecular weight.

Description: A primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na²⁺ retention potency.

Water Soluble Hydrocortisone: 2-hydroxypropyl-beta-cyclodextrin is a ring structure which is soluble in water to approximately 50% (w/v). This ring forms a "cavity" in the center of the molecule in which hydrophobic compounds like hydrocortisone can "fit". Hydrophobic molecules are incorported into the cavity of cyclodextrins by displacing water. This reaction is favored by the repulsion of the molecule by water. This effectively encapsulates the molecule within the cyclodextrin, rendering the molecule water soluble. When the water soluble complex is diluted in a much larger volume of aqueous solvent, the process is reversed, thereby releasing the molecule of interest into the solution.

Availability:
 

References:
 

1. Merck Index 12th Ed., No. 4828.
2. Hoegger, Petra, "Comparison of the tissue affinity of glucocorticoids to human lung, nasal and skin tissue in vitro." Arzneim. Forsch., v. 51(10), 825-831 (2001).
3. McEwen, B.S., et al., "Adrenal steroid receptors and actions in the nervous system." Physiol. Rev., v. 66, 1121 (1986).
4. Parrillo, J.E. and Fauci, A.S., "Mechanisms of glucocorticoid action on immune processes." Ann. Rev. Pharmacol. Toxicol., v. 19, 179 (1979).

原厂资料：