NP MIDI 4-12BT GEL, 20W BOX OF 10 GELS + MANUAL

• 产品编号：WG1402BOX      品牌：invitrogen       原厂货号：WG1402BOX
• 产品分类：蛋白质检测与分析 > 蛋白质凝胶电泳 > 预制蛋白胶 > Bis-Tris胶
• 应用分类：

描述：

NuPAGE® Novex Bis-Tris Gels provide the best separation and resolution of small- to medium-sized proteins by utilizing a neutral pH environment which minimizes protein modifications. These high-performance gels have a one-year shelf life when stored at +4°C to 25°C, eliminating the waste of throwing out expired gels. Use NuPAGE® Novex Bis-Tris Gels for protein sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. The NuPAGE® System also provides the most efficient means for transferring proteins to membranes for subsequent analysis.

NuPAGE® Novex Bis-Tris Gels are now available in the midi-format (8 x 13 cm, L x W). The NuPAGE® Midi-Gels are run in the XCell4 Surelock™ Midi-Cell that can run up to 4 gels at a time. Each Midi-Gel can run up to 26 samples, so that over 100 samples can be run at a time. With a Midi Gel Adapter, Midi-Gels can run in a Bio-Rad Criterion™ Cell.

Formulation: The NuPAGE® System is based upon a Bis-Tris-HCl buffered (更看更多pH 6.4) polyacrylamide gel, with a separating gel that operates at pH 7.0. While NuPAGE® Novex Bis-Tris Gels do not contain SDS, they are formulated for denaturing gel electrophoresis applications only.


Recommended Buffers: NuPAGE® Novex Bis-Tris Gels are available in three acrylamide concentrations: 8, 10%, and 4-12%. By combining any of these three gel types with the NuPAGE® MES or MOPS Buffers, six separation ranges can be obtained. The NuPAGE® MES Buffer is recommended for resolving small proteins, while NuPAGE® MOPS Buffer is for resolving medium to large size proteins. Use of NuPAGE® LDS Sample Buffer and NuPAGE® Sample Reducing Agent will ensure complete sample reduction. For transfer, NuPAGE® Transfer Buffer is recommended.

12+2 wells
Maximum sample volume/well^‡ 45 µl sample + 15 µl standards
Maximum protein load Coomassie® Stain 0.7 µg/band
Maximum protein load Silver Stain 1.4 ng/band

20 wells
Maximum sample volume/well^‡ 25 µl
Maximum protein load Coomassie® Stain 0.5 µg/band
Maximum protein load Silver Stain 1 ng/band

26 wells
Maximum sample volume/well^‡ 15 µl
Maximum protein load Coomassie® Stain 0.35 µg/band
Maximum protein load Silver Stain 0.7 ng/band

Currently using: Recommended NuPAGE® Gel:
4% Tris-Glycine NuPAGE® 3-8% Tris-Acetate (+ TA Buffer)
6% Tris-Glycine NuPAGE® 3-8% Tris-Acetate (+ TA Buffer)
10% Tris-Glycine NuPAGE® 8% Bis-Tris (+ MOPS Buffer)
or NuPAGE® 10% Bis-Tris (+ MOPS Buffer)
12% Tris-Glycine NuPAGE® 10% Bis-Tris (+ MOPS Buffer)
4-12% Tris-Glycine NuPAGE® 3-8% Tris-Acetate (+ TA Buffer)
or NuPAGE® 4-12% Bis-Tris (+ MOPS Buffer)
4-20% Tris-Glycine NuPAGE® 4-12% Bis-Tris (+ MES Buffer)
8-16% Tris-Glycine NuPAGE® 4-12% Bis-Tris (+ MOPS Buffer)
10% Tricine NuPAGE® 8% Bis-Tris (+ MES Buffer)
or NuPAGE® 10% Bis-Tris (+ MES Buffer)
16% Tricine NuPAGE® 4-12% Bis-Tris (+ MES Buffer)
10-20% Tricine NuPAGE® 4-12% Bis-Tris (+ MES Buffer)


For more information on the benefits of NuPAGE neutral pH technology go to www.invitrogen.com/nupage

 

原厂资料：

注意事项：

For Research Use Only. Not for use in diagnostic procedures.