The amine-reactive Oregon Green® 514 succinimidyl ester can be used to create green fluorescent bioconjugates with excitation/emission maxima ~489/526 nm. This fluorinated analog of fluorescein overcomes some of the key limitations of fluorescein, including greater photostability and a lower pKa, making its fluorescence essentially pH insensitive in the physiological pH range.
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For Research Use Only. Not for use in diagnostic procedures.