PMIR-REPORT REPORTER VECTOR 0.5ML

• 产品编号：AM5795      品牌：invitrogen       原厂货号：AM5795
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi载体（shRNA)
• 应用分类：

描述：

The Ambion® pMIR-REPORT™ miRNA Expression Reporter Vector System is for the measurement of miRNA expression in cells. It contains two mammalian expression vectors-—one for cloning miRNA targets and evaluating miRNA regulation, and the other for the normalization of transfection efficiency.

• Clone miRNA targets and evaluate miRNA regulation
• Screen putative miRNA target sequences
• Includes both reporter and control vectors

The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell measurement of miRNA expression. This validated reporter system contains two mammalian expression vectors. The pMIR-REPORT™ Luciferase miRNA Expression Reporter Vector contains firefly luciferase under the control of a mammalian promoter⁄terminator system, with an miRNA target cloning region downstream of the luciferase translation sequence. This vector is optimized for cloning miRNA targets and evaluating miRNA regulation. A second vector, pMIR-REPORT™ Beta-查看更多galactosidase Reporter Control Vector, is provided for normalizing transfection efficiency.

Applications:
pMIR-REPORT™ Luciferase is designed for cloning and testing putative miRNA binding sites. pMIR-REPORT™ Luciferase can be transfected into mammalian cells to evaluate endogenous miRNA expression, or used to evaluate the up- or down-regulation resulting from the transfection of Pre-miR™ miRNA Molecules or Anti-miR™ miRNA Inhibitor Molecules, respectively.

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