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4-硝基苯磷酸二钠P-Nitrophenyl Phosphate, Disodium Salt Hexahydrate

 
包装: 1 g
运保温度: 0°C
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描述:

4-Nitrophenyl phosphate disodium salt hexahydrate; di-Sodium 4-nitrophenyl phosphate; Disodium 4-nitrophenyl phosphate hexahydrate; pNPP disodium hexahydrate

Disodium Salt
Hexahydrate
Purity: 99+%
White to pale yellow crystals
Free p-nitrophenol <0.005%
Suitable for use as a substrate for alkaline and acid phosphatase

 

Description: Suitable of use as a substrate for alkaline and acid phosphatase.
pNPP is the substrate of choice for use with alkaline phosphatase in ELISA procedures. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 M NaOH and read at 405 nm.

中文名称: 对硝基苯磷酸二钠

中文别名: PNPP,4-硝基苯磷酸二钠,磷酸-4-硝基苯酯二钠盐
英文名称: PNPP

英文别名: Disodium 4-nitrophenyl phosphate

    用途:测定磷酸酶的显色底物;测定酸性和碱性磷酸酶(phosphatases)的显色底物;反应能产生黄色的末端产物,在波长405nm可以读取,该反应能被3 N NaOH溶液终止。


注意事项:

Buffer Preparation:
To prepare 0.1 M glycine buffer, 1 mM MgCl2, 1 mM ZnCl2, pH 10.4: Add 7.51 g of glycine (MP catalog number 808822, 808831), 203 mg MgCl2 (MP catalog number #195304) and 136 mg ZnCl2 (MP catalog number 193899) to approximately 980 ml deionized water and mix. Adjust pH to 10.4 with 19 N NaOH and QS to 1 L with deionized water.
To prepare 1 M diethanolamine buffer 0.5 mM MgCl2, pH 9.8: Add 97 ml diethanolamine (MP catalog number 150166), 100 mg MgCl2, and 0.2 g sodium azide (MP catalog number 102891) to 800 ml deionized water, adjust pH to 9.8 with 10 M HCl and QS to 1 L with deionized water.


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