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注意事项:
Buffer Preparation:
To prepare 0.1 M glycine buffer, 1 mM MgCl2, 1 mM ZnCl2, pH 10.4: Add 7.51 g of glycine (MP catalog number 808822, 808831), 203 mg MgCl2 (MP catalog number #195304) and 136 mg ZnCl2 (MP catalog number 193899) to approximately 980 ml deionized water and mix. Adjust pH to 10.4 with 19 N NaOH and QS to 1 L with deionized water.
To prepare 1 M diethanolamine buffer 0.5 mM MgCl2, pH 9.8: Add 97 ml diethanolamine (MP catalog number 150166), 100 mg MgCl2, and 0.2 g sodium azide (MP catalog number 102891) to 800 ml deionized water, adjust pH to 9.8 with 10 M HCl and QS to 1 L with deionized water.
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