The S.N.A.P.™ MidiPrep Kit is a medium-scale plasmid DNA purification kit designed for fast and easy purification of pure plasmid DNA from 10-100 ml of overnight culture (Figure 1). The S.N.A.P.™ MidiPrep procedure takes just 40 minutes. You simply:
Lyse cells and filter debris with the S.N.A.P.™ filtering column
Apply flow-through to the S.N.A.P.™ DNA binding column
Wash the column
Elute pure, supercoiled plasmid DNA in water or TE buffer
Plasmid DNA purified with the S.N.A.P.™ MidiPrep Kit is ready to use right off the column. No precipitation is necessary. Plasmid is eluted in TE or water at ~200 ng/µl-perfect for subsequent applications including mammalian cell transfection.
注意事项:
For Research Use Only. Not for use in diagnostic procedures.