TaqMan®Universal Master Mix II 可在较宽的靶基因输入量范围内完成敏感且精确的检测，它可以可靠地检测出低拷贝数靶点，并通过准确定量鉴别出靶点丰度之间的微小差异。与所有基于TaqMan®Assay的技术一样，此实时荧光定量 PCR预混液提供了同源序列之间的单碱基分辨，且可在通用热循环条件下完成反应。
原厂资料：
TaqMan® Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). You can use TaqMan® Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.
This TaqMan® Universal Master Mix II is supplied as a 2X concentrate and contains:
• AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure) • dNTPs (with dUTP) • ROX™ Passive Reference • Optimized buffer components
Note:This TaqMan® Universal Master Mix II does not contain Uracil-N glycoslyase (UNG). See a complete listing of all TaqMan® Universal Master Mix II products, with and without UNGhere.
TaqMan® Universal Master Mix II has been optimized for use with primers and TaqMan® probes that have been designed according to Life Technologies' guidelines. The master mix can be used with custom TaqMan® assays available from our custom assay service, or with pre-optimized assays such as:
• TaqMan® Gene Expression Assays • TaqMan® MicroRNA Assays • TaqMan® Drug Metabolism Genotyping Assays • TaqMan® SNP Genotyping Assays
For RNA quantitation experiments, the TaqMan® Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction usingcDNA synthesis kitsavailable from Life Technologies.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
