FG,TAQMAN GT MASTER MIX,50 MLEACH

• 产品编号：4371357      品牌：invitrogenGA       原厂货号：4371357
• 产品分类：DNA和RNA纯化 > 核酸分析 > 基因型分析 > SNP（单核苷酸多态性）分析
• 应用分类：

描述：

The Applied Biosystems® TaqMan® Genotyping Master Mix is optimized for end-point fluorescence detection in SNP genotyping applications.

• Distinct clusters and high call rates for unambiguous allelic discrimination
• Validated with TaqMan® SNP Genotyping Assays
• Excellent pre- and post-PCR stability for high throughput setup and analysis

May be used for a variety of genotyping applications, including:

• Candidate gene studies
• Drug-target validation
• Disease association studies
• Population genetics
• Linkage mapping
• Agricultural applications

Reaction size is based on a 50 µl reaction volume; please see below for other reaction sizes.

Exceptional Performance from an Optimized Mix (2X)
Accurate genotype assignments result from preferential binding of the allele-specific probe to the matching target. TaqMan® Genotyping Master Mix enables specific binding of the probe to achieve exceptional cluster resolution. Key features include:

• AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure) – Automatic hot start enzyme designed to be active during thermal cycling and inactive at room temperature for easy reaction setup.
• Optimized mix components provide excellent specificity for discrimination between alleles
• Passive internal reference based on proprietary ROX™ dye for increased precision on Applied Biosystems® real-time PCR instruments
• Single thermal cycling condition for consistent results with TaqMan® Assays

Validated with TaqMan® Genotyping Assays and Instruments
TaqMan® Genotyping Master Mix has been tested across all types of TaqMan® SNP Genotyping Assays: TaqMan® Drug Metabolism Genotyping Assays, TaqMan® SNP Genotyping Assays, and Custom TaqMan® SNP Genotyping Assays. In addition, the master mix is validated with the Applied Biosystems® thermal cyclers and real-time PCR systems.

High-Throughput Setup and Analysis
The combination of several low-cost thermal cyclers for PCR and a single real-time PCR instrument for allelic discrimination make high throughput SNP genotyping manageable. TaqMan® Genotyping Master Mix further improves high-throughput setup and analysis with excellent room temperature stability both before and after PCR. This provides the flexibility required to run long experiments, unattended overnight or over a weekend.

Reliable Discrimination with Challenging Targets
Amplicon design constraints make SNP detection challenging, particularly when targets contain an abundance of GC- or AT-rich regions. The optimized components of TaqMan® Genotyping Master Mix result in tight, well-separated clusters and more accurate allele-calls compared to other mixes, even for challenging targets.

Setting the Industry Standard for Quality
Extensive analytical and functional tests are performed for each manufactured lot of TaqMan® Genotyping Master Mix, including one TaqMan® SNP Genotyping Assay and one TaqMan® Drug Metabolism Genotyping Assay. Results are compiled in an informative Certificate of Analysis, which is accessible through the Applied Biosystems® Web site (search for 'Certificate of Analysis').

Number of Genotyping Reactions at a Given Reaction Size:

4371355, TaqMan® Genotyping Master Mix, 1-Pack (1 x 10 mL)
• 50 µL Reactions: 400
• 20 µL Reactions: 1000
• 10 µL Reactions: 2,000
• 5 µL Reactions: 4,000

4381656, TaqMan® Genotyping Master Mix, 2-Pack (2 x 10 mL)
• 50 µL Reactions: 800
• 20 µL Reactions: 2,000
• 10 µL Reactions: 4,000
• 5 µL Reactions: 8,000

4371357, TaqMan® Genotyping Master Mix, (1 x 50 mL)
• 50 µL Reactions: 2000
• 20 µL Reactions: 5,000
• 10 µL Reactions: 10,000
• 5 µL Reactions: 20,000

4381657, TaqMan® Genotyping Master Mix, (2 x 50 mL)
• 50 µL Reactions: 4000
• 20 µL Reactions: 10,000
• 10 µL Reactions: 20,000
• 5 µL Reactions: 40,000

 

原厂资料：

注意事项：

For Research Use Only. Not for use in diagnostics procedures