Cell Proliferation Kit

• 产品编号：BD-562253      品牌：BD-Pharmingen       原厂货号：562253
• 产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞增殖及周期检测试剂盒
• 应用分类：

其它组分：

描述：

Multiparameter flow cytometry provides a powerful tool for resolving mechanisms by which individual cells in homogenous or mixed cell
populations maintain viability, enter and progress through cell cycle or undergo cell death. For this purpose, the Apoptosis, DNA Damage, and
Cell Proliferation Kit was designed with the inclusion of fluorescent antibodies specific for incorporated BrdU, phosphorylated H2AX
(γH2AX) and cleaved PARP. These probes along with optimized protocols enable multicolor flow cytometric analysis of proliferation, DNA
damage and apoptosis, respectively, by individual cells within samples.
Immunofluorescent staining of cells that have incorporated Bromodeoxyuridine (BrdU, an analog of the DNA precursor thymidine) and flow
cytometric analysis provides a high resolution technique to determine the frequency and nature of individual cells that have synthesized DNA.
Exposure of cells to BrdU in either in vitro or in vivo experimental model systems allows for BrdU incorporation by actively cycling cell
fractions. Pulse labeling of cells with BrdU at various time points, permits the determination of cell-cycle kinetics. Flow cytometric analysis of
BrdU incorporation can readily be combined with the simultaneous analysis of cellular phosphorylated H2AX and cleaved PARP levels.
Phosphorylated H2AX functions to recruit and localize DNA repair proteins or cell cycle checkpoint factors to DNA-damaged sites. In this
way, phosphorylated H2AX promotes DNA repair and maintains genomic stability. Double-stranded DNA breaks caused by replication errors,
apoptosis, or other physiological processes (including, immunoglobulin and TCR gene recombinations) and DNA damage caused by ionizing
radiation, UV light, or cytotoxic agents lead to H2AX phosphorylation on serine 139, H2AX (pS139), to induce its function. PARP (Poly
[ADP-Ribose] Polymerase) is a nuclear chromatin-associated enzyme that is involved in DNA repair. During apoptosis, Caspase-3 cleaves
PARP resulting in its inactivation and the inability of cells to repair DNA damage. For this reason, the 89 kDa-cleaved fragment of PARP
serves as a marker of cellular apoptosis.
 

原厂资料：

注意事项：

The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were
removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.