APO-BrdU Apop Detection FITC Kit

包装：

Kit

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其它组分：

APO-BRDU™ Kit Part A【子货号：51-6576AK，，运保温度：should be stored at 4°C upon arrival】

APO-BRDU™ Kit Part B【子货号：51-6576BK，，运保温度：dry ice】

描述：

One of the later steps in apoptosis is DNA fragmentation, a process which results from the activation of endonucleases during the apoptotic

program. These nucleases degrade the higher order chromatin structure into fragments of ~300 kb and subsequently into smaller DNA pieces

of about 50 bp length. A method which is often used to detect fragmented DNA utilizes a reaction catalyzed by exogenous TdT, often referred

to as "end-labeling" or "TUNEL" (terminal deoxynucleotidyltransferase dUTP nick end labeling). In the APO-BRDU™ assay, TdT catalyzes a

template-independent addition of bromolated deoxyuridine triphosphates (Br-dUTP) to the 3'-hydroxyl (OH) termini of double- and

single-stranded DNA. After incorporation, these sites are identified by flow cytometric means by staining the cells with a FITC-labeled

anti-BrdU mAb. The APO-DIRECT™ assay (Cat. No. 51-6536KK) is a single-step method for labeling DNA breaks with FITC-dUTP,

followed by flow cytometric analysis.

The APO-BRDU™ Kit is a two color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow

cytometry. Sufficient reagents are provided to process 60 cell suspensions. The kit includes 5 ml of positive and 5 ml of negative control cell

suspensions, approximately 1 x 10^6 cells per ml in 70% (v/v) ethanol. The control cells are derived from a human lymphoma cell line and

have been fixed as described in the Fixation Protocol.

注意事项：

Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before

discarding to avoid accumulation of potentially explosive deposits in plumbing.