APO-Direct Apop Detection Kit

• 产品编号：BD-556381      品牌：BD-Pharmingen       原厂货号：556381
• 产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞凋亡试剂盒
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描述：

One of the later steps in apoptosis is DNA fragmentation, a process which results from the activation of endonucleases during the apoptotic program. These nucleases degrade the higher order chromatin structure into fragments of ~300 kb and subsequently into smaller DNA pieces of about 50 bp in length. A method which is often used to detect fragmented DNA utilizes a reaction catalyzed by exogenous TdT, often referred to as "end-labeling" or "TUNEL" (terminal deoxynucleotidyltransferase dUTP nick end labeling). The APO-DIRECT™ assay (Cat. No. 6536KK) is a single-step method for labeling DNA breaks with FITC-dUTP, followed by flow cytometric analysis. In the APO-BRDU™ assay, TdT catalyzes a template-independent addition of bromolated deoxyuridine triphosphates (Br-dUTP) to the 3'-hydroxyl (OH) termini of double- and single-stranded DNA. After incorporation, these sites are identified by flow cytometric means by staining the cells with a FITC-labeled anti-BrdU mAb.

APO-DIRECT™ is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry. Sufficient reagents are provided to process 50 tests. The kit includes 5 ml of positive and 5 ml of negative control cell suspensions, approximately 1 x 10^6 cells per ml in 70 % ( v/v) ethanol. The control cells are derived from a human lymphoma cell line and have been fixed as described below in the Fixation Protocol.

原厂资料：

One of the later steps in apoptosis is DNA fragmentation, a process which results from the activation of endonucleases during the apoptotic program. These nucleases degrade the higher order chromatin structure into fragments of ~300 kb and subsequently into smaller DNA pieces of about 50 bp in length. A method which is often used to detect fragmented DNA utilizes a reaction catalyzed by exogenous TdT, often referred to as "end-labeling" or "TUNEL" (terminal deoxynucleotidyltransferase dUTP nick end labeling). The APO-DIRECT™ assay (Cat. No. 6536KK) is a single-step method for labeling DNA breaks with FITC-dUTP, followed by flow cytometric analysis. In the APO-BRDU™ assay, TdT catalyzes a template-independent addition of bromolated deoxyuridine triphosphates (Br-dUTP) to the 3'-hydroxyl (OH) termini of double- and single-stranded DNA. After incorporation, these sites are identified by flow cytometric means by staining the cells with a FITC-labeled anti-BrdU mAb.

APO-DIRECT™ is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry. Sufficient reagents are provided to process 50 tests. The kit includes 5 ml of positive and 5 ml of negative control cell suspensions, approximately 1 x 10^6 cells per ml in 70 % ( v/v) ethanol. The control cells are derived from a human lymphoma cell line and have been fixed as described below in the Fixation Protocol.

The APO-DIRECT™ Kit consists of two parts (part A and B).

Part A (Comp. No. 51-6536AK) consists of:

One 25 ml bottle of PI/RNase Staining Buffer (5 µg/ml, 200 µg/ml RNase) [Comp. No. 51-6551AZ],

One 0.5 ml vial of Reaction Buffer (containing cacodylic acid (dimethylarsenic)) [Comp. No. 51-6549AZ ] ,

One 100 ml bottle of Rinsing Buffer (containing 0.05% sodium azide) [Comp. No. 51-6550AZ]

One 100 ml botle of Wash Buffer (containing 0.05% sodium azide) [Comp. No. 51-6548AZ].

Part B (Comp. No. 51-6536BK) consists of:

One 0.4 ml bottle of FITC-dUTP (0.25 nMol) [Comp. No. 51-6555EZ]

One 5 ml bottle of Negative Control Cells [contains 70% (v/v) ethanol] [Comp. No. 51-6553LZ]

One 5 ml bottle of Positive Control Cells [contains 70% (v/v) ethanol] [Comp. No. 51-6552LZ]

One 0.038 ml bottle of TdT Enzyme [200 µg/ml (S.A.= 100,000 U/mg) in 50% (v/v) glycerol solution; will not freeze at -20°C] [Comp. No. 51-6554EZ].

注意事项：