MiCK-2 Cytokine Positive Control Cells

产品编号：BD-554653 品牌：BD-Pharmingen 原厂货号：554653
产品分类：细胞,细胞培养与转染试剂 > 细胞 > 哺乳动物细胞系
应用分类：

包装：

Cells

运保温度:

Store product at -80°C prior to use or for long te

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描述：

This suspension contains Mouse intracellular CytoKine-2 (MiCK-2) Positive Control Cells. The MiCK-2 cell suspension contains fixed,
non-permeabilized mouse lymphoid cells (20 test samples at 2.5 x 10^5 cells/test) in 1 mL final volume. The suspension includes cells that
express easily detectable levels of intracellular IL-4, IL-10 or GM-CSF as determined by immunofluorescent staining of intracellular cytokines
and flow cytometry. MiCK-2 cell suspensions were prepared by stimulating mouse spleen cells in the presence of a protein transport inhibitor.
After stimulation, the cells were harvested and were incubated with BD FcBlock™ [rat IgG2b, κ anti-mouse CD16/CD32 (FcγII/III receptor)
antibody; Cat. No. 553142] to reduce Fc receptor-mediated background staining. The cells were fixed and then stored in 10%
dimethylsulfoxide (DMSO) and 90% fetal bovine serum at -80°C.

注意事项：

Store product at -80°C prior to use or for long term storage of stock solutions.
Rapidly thaw and quick-spin product prior to use.
Avoid multiple freeze-thaws of product.
This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Upon receipt, store the cell suspension at -80°C. Alternatively, the frozen cell suspension can be thawed and the capped vial
should be quick spun to ensure complete retrieval of the contents from the vial. After thoroughly resuspending cells with a
pipette, "single-use" aliquots can be refrozen and stored (-80°C) in polypropylene microtubes for a use at a later time.