Hu IL-12 p70 Recom 5ug

产品编号：BD-554613 品牌：BD-Pharmingen 原厂货号：554613
产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IL-12 Family 白细胞介素-12超家族
应用分类：细胞培养产品与服务 > 哺乳动物细胞培养 > 生长因子和纯化蛋白质 > 物种 > 人
　　　　　细胞培养产品与服务 > 哺乳动物细胞培养 > 细胞因子 > 白细胞介素

包装：

5ug

运保温度:

stored at –80°C.

到货周期：

登录后查看

标准价：

￥客户可见

会员价：

￥客户可见

积分：

客户可见分

登录之后可加入购物车购买，请您

运费与支付说明：

1.含干冰类产品有运费；

2. 必须现金付款或有信用额度的会员才可以直接发货，否则需要等待现金付款信息。

描述：

Interleukin-12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. The IL-12 p40 monomer shares amino acid sequence homology with the IL-6 receptor. It has been reported that activated PBMC produce a manyfold excess of IL-12 p40 monomer over the bioactive p70 heterodimer. The IL-12 p40 monomer has been reported to inhibit binding of IL-12 p70 to the IL-12 receptor, but with 20X less effectiveness than the IL-12 p70 homodimer.

Formulation and Purity:

Recombinant human IL-12 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution containing 1 mg/ml Biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Purity is > 95% as determined by non-reducing SDS-PAGE, and an absorbance assay based on the Beers-Lambert Law. The endotoxin level is ≤ 0.1 ng per μg of human IL-12 p70, as measured in a chromogenic LAL assay.

注意事项：

Store product at -80°C prior to use or for long term storage of stock solutions.
Rapidly thaw and quick-spin product prior to use.
Avoid multiple freeze-thaws of product.
This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use.
Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as
human or bovine albumin, aliquoted and stored at –80°C. Carrier proteins should be pre-screened for possible effects in an
appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or
possible blocking activity. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1 mg/ml. For
use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at
indicated temperatures may result in a loss of activity. The product should not be diluted to less than 25 μg/ml for long term
storage. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins
may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.