Rb Undrphos PE Set G99-549 100Tst

• 产品编号：BD-550502      品牌：BD-Pharmingen       原厂货号：550502
• 产品分类：抗体 > 一抗 > 抗体对
• 应用分类：

原厂资料：

The retinoblastoma gene encodes a nuclear phosphoprotein (Rb or p110Rb) which is expressed in most normal cells of vertebrates and acts as
a tumor suppressor. The underphosphorylated form of Rb is mainly found in resting or fully differentiated cells, whereas the more highly
phosphorylated forms are present in proliferating cells. Thus, the underphosphorylated form of Rb can act as a marker of cells in G0/G1 phase
of the cell cycle. Growth factors act to promote cells through the G1 and S phase of the cell cycle and during this time Rb undergoes
sequential phosphorylation. Rb can act as a gatekeeper to regulate the cell cycle because in the underphosphorylated form it binds to critical
regulatory proteins including E2F which acts to repress transcription. When Rb becomes phosphorylated, E2F is released and transcription can
occur. Disregulation of the cell cycle can be caused by mutations in Rb as well as in p53, which can lead to unregulated growth or
oncogenesis. The expression of underphosphorylated Rb has been found to be unregulated following induction of cell cycle arrest. There are
numerous methods to induce cell cycle arrest and a typical model system is the use of TPA to induce cell cycle arrest in MOLT-4 cells. Clone
G99-549 has been reported to recognize only the underphosphorylated form of the human Rb protein. The epitope for clone G99-549 has been
reported to be mapped to amino acids 514-610 of human Rb.

注意事项：

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
5.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.