A peptide corresponding to amino acids 177-189 of Q9Y478.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS
Preservative
0.09% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Flow Cytometry (Flow)
0.5-1 ug/test
Immunocytochemistry (ICC)
4-6 ug/ml
Immunofluorescence (IF)
4-6 ug/ml
Immunohistochemistry (Paraffin) (IHC (P))
4-6 ug/ml
Western Blot (WB)
2-3 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
This antibody is predicted to react with bovine, canine, carp, chicken, equine, mouse, opossum, orangutan, platypus, rat, salmon, porcine, Xenopus and zebrafish based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
AMPKa belongs to the family of AMP activated protein kinases, that plays a central role in regulating cellular and organismal energy balance in response to the balance between AMP/ATP, and intracellular Ca2+ levels. AMPK exists as a heterotrimer comprising of catalytic alpha subunit, and regulatory beta and gamma subunits. The alpha subunit is activated by increases in the cellular AMP:ATP ratio caused by metabolic stresses, and can mediate the effects of the adipokines leptin and adiponectin. Two isoforms differ in subcellular localization and AMP-dependence. Phosphorylation of threonine 172 in the alpha subunit is a key determinant of AMPK activity.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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