Lyophilized powder. Avidin is supplied as salt-free protein. Reconstitute with deionized water.
DESCRIPTION:
Avidin is chromatographically purified from egg white. The extinction coefficient,E(1%) at 280nm is 15.5.
CAPACITY:
Binding activity of avidin is determined to be 11-15 units per mg protein. One unit of binding activity is defined as that amount of protein which binds 1 μg of biotin (Green, N.M.,chem.J.,89 :599, 1963).
APPLICATIONS:
Avidin can be used as linker in biotin-avidin techniques. It can be used to coat microtiter plate, conjugate to enzymes, fluorochromes or other signal generating tags.