raised in rabbits against the C-terminal region of human HDAC1, using a KLH-conjugated synthetic peptide.
Conjugate
Unconjugated
Form
Frozen
Concentration
Lot-Specific
Purification
Affinity chromatography
Preservative
<0.1% sodium azide
Storage Conditions
-20° C, Avoid Freeze/Thaw Cycles
Tested Applications
Dilution *
ChIP assay (ChIP)
2.4 ug
ELISA (ELISA)
1:100 to 1:300
Immunocytochemistry (ICC)
1:200
Immunofluorescence (IF)
1:200
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p. HDAC1 is a class I histone deacetylase containing 482 amino acid residues. HDAC1 has been shown to interact directly with transcription factors and has been shown to deacetylate histone proteins H3 and H4.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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