Neurobasal® Medium is a basal medium that meets the special cell culture requirements of pre-natal and embryonic neuronal cells when used with GIBCO® B-27® Supplement. Neurobasal® Medium can be used to grow neuronal cells from hippocampus, cortex and other regions of the brain. Neurobasal® Medium allows for both long and short term maintenance of homogeneous populations of neuronal cells without the need of an astrocyte feeder layer.
Add Your Own Supplements
All Neurobasal® media contain no L-glutamine, L-glutamic acid, or aspartic acid. Neurobasal® media must be supplemented with 0.5 mM L-glutamine or GlutaMAX™-I Supplement and either a serum-free supplement (such as B-27® Supplement or N-2 Supplement), or with serum. For initial plating, you should add 25 µM glutamic acid.
Quality Control Testing
All Neurobasal® media are tested for pH, osmolality, endotoxin, and the absence of bacterial and fungal contamination.
Neurobasal® Medium contains Phenol Red. If Phenol Red will interfere with your applications, we also provide Neurobasal® Medium Minus Phenol Red.
Neurobasal® Medium is optimized for pre-natal and embryonic neurons. For growing post-natal and adult brain neurons, we provide Neurobasal®-A Medium.
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