A 27 amino acid synthetic peptide derived from the MH1 domain of human Smad2. This peptide shares 24/27 residues with the mouse Smad2 protein and 26/27 residues with the Xenopus Smad2 protein.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.25 mg/ml
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Preservative
0.1% sodium azide
Storage Conditions
-20°C
Tested Applications
Dilution *
ChIP assay (ChIP)
1-3 µg x 10^6 cells
ELISA (ELISA)
0.1-1.0 ug/ml
Flow Cytometry (Flow)
3-5 µg x 10^6 cells
Immunohistochemistry (Paraffin) (IHC (P))
1:20-1:200
Western Blot (WB)
1-3 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
SMAD2, also known as MADH2 or MAD2 regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. Smad2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signalregulated kinase 1 (ERK1) enhances Smad2 transcriptional activity, which is negatively regulated by calmodulin.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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