Advanced MEM (Minimum Essential medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic MEM, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.i Cells successfully cultured in Advanced DMEM ⁄ F-12, with no adaptation, include PK-15, MDCK, Vero, WI-38 and Hep-2.
This Advanced MEM is manufactured as follows:
With
Without
• Glucose
• L-glutamine
• Non Essential Amino Acids
• HEPES
• Sodium pyruvate
• Phenol Red
The complete formulation is available.
Gibco® Advanced MEM is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX® I lipid-rich bovine serum albumin for 查看更多cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.
Product Intended Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
cGMP Manufacturing and Quality System
Gibco® Advanced MEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 and ISO 9001 standards.
Advanced MEM requires supplementation with 1-5% Fetal Bovine Serum and 4mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g ⁄ L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
iPaul Price, Brad Stiles, David Staines, Ethel Evege, Femi Fatunmbi, Kate Wagner, Lori Nestler, David Jayme. Advanced Media as Alternatives to Classical Basal Media. FOCUS, 2003. 25.2:3-6.
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