CMRL medium was originally developed by Connaught Medical Research Laboratories for the growth of Earle's "L" cells under serum-free conditions. CMRL medium is also especially useful for cloning monkey kidney cells and for growth of many other mammalian cell lines when supplemented with horse or calf serum.
Gibco® CMRL medium is a modified version of Medium 199. CMRL medium is particularly rich in nucleosides and vitamins
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.
Dual-Site cGMP Manufacturing
Gibco® CMRL medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® CMRL medium product made in our Scotland facility (21530-027). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
CMRL medium contains no proteins or growth factors. Therefore, CMRL medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). CMRL medium uses a sodium bicarbonate buffer system (2.2 g ⁄ L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
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