A-TRIMETHYL-HIST H3 (LYS4)

• 产品编号：merck-m-07-473      品牌：millipore       原厂货号：07-473
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Anti-trimethyl-Histone H3 (Lys4) 抗体即Anti-H3K4me3是兔多克隆抗体用于检测组蛋白H3第四位赖氨酸的三甲基化。

应用：ChIP, DB, WB, PIA, ChIP-seq.

原厂资料：

Anti-trimethyl-Histone H3 (Lys4) Antibody.

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Key Applications

• Chromatin Immunoprecipitation (ChIP)
• Dot Blot
• Western Blotting
• ChIP-seq

注意事项：

ChIP Analysis: 
An independent lab has shown that this antibody performs in chromatin immunoprecipitation (ChIP).

ChIP-seq Analysis: 
Chromatin immunoprecipitation was performed using the Magna ChIP™ HiSens kit (cat# 17-10460), 1 µL Anti-trimethyl-Histone H3 (Lys4) antibody (cat# 07-473) , 20 µL Protein A/G beads, and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols with Illumina barcoded adapters, and analyzed on Illumina HiSeq instrument. An excess of eighteen million reads from FastQ files were mapped using Bowtie (http://bowtie-bio.sourceforge.net/manual.shtml) following TagDust (http://genome.gsc.riken.jp/osc/english/dataresource/) tag removal. Peaks were identified using MACS (http://luelab.dfci.harvard.edu/MACS/), with peaks and reads visualized as a custom track in UCSC Genome Browser (http://genome.ucsc.edu) from BigWig and BED files. The highest 25% of peaks identified in the 07-473 dataset showed 99% overlap with peaks identified in the ENCODE H3K4me3 BROAD Histone track for HeLa S3.