small nuclear ribonucleoprotein 70kDa (RNP antigen)
U1 small nuclear ribonucleoprotein 70 kDa
Background Information
U1 small nuclear ribonucleoprotein 70 kDa (U1-70K) is one of three specific proteins found within the U1 snRNP core, which includes at least seven core proteins found in all U snRNPs and three specific proteins; U1-70K, U1-A, and U1-C. U1-70K is involved in mediating pre-mRNA splicing through binding the loop I region of U1-snRNA with its RPM (RNA recognition motif) binding domain. In addition, its RNA binding domain, U1-70K also contains two argine-rich regions, accented with acidic or serine residues, that is thought to have trans-acting functions. Alternative splicing results in 4 known isoforms. Truncated forms of U1-70K are unable to bind RNA.
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Use Anti-U1-70K Antibody, clone 9C4.1 (Mouse Monoclonal Antibody) validated in WB, ICC to detect U1-70K also known as small nuclear ribonucleoprotein 70kDa (U1).
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:500 dilution of a representative lot of this antibody has been shown to detect U1-70K in NIH/3T3, A431, and HeLa cells.
Biological Information
Immunogen
Recombinant protein corresponding to C-terminal domain of human U1-70K.
Epitope
C-terminal domain
Clone
clone 9C4.1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes U1-70K at the C-terminal domain.
FUNCTION: Mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA.
SUBUNIT STRUCTURE: Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B/SFRS10. Interacts with dephosphorylated SFRS13A and SFPQ. Interacts with NUDT21/CPSF5, CPSF6, SFRS2IP, and ZRANB2.
SUBCELLULAR LOCATION: Nucleus.
PTM: The N-terminus is blocked.
Extensively phosphorylated on serine residues in the C-terminal region.
MISCELLANEOUS: Major ribonucleoprotein antigen recognized by the sera from patients with autoimmune diseases, such as systemic lupus erythematosus.
SEQUENCE CAUTION: The sequence CAA28352.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA29964.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA29966.1 differs from that shown. Reason: Erroneous initiation.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected U1-70K on 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H, R, M
WB, ICC
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1588
Description
Anti-U1-70K Antibody, clone 9C4.1
Alternate Names
small nuclear ribonucleoprotein 70kDa (U1)
U1 snRNP 70 kDa
small nuclear ribonucleoprotein 70kDa (RNP antigen)
U1 small nuclear ribonucleoprotein 70 kDa
Background Information
U1 small nuclear ribonucleoprotein 70 kDa (U1-70K) is one of three specific proteins found within the U1 snRNP core, which includes at least seven core proteins found in all U snRNPs and three specific proteins; U1-70K, U1-A, and U1-C. U1-70K is involved in mediating pre-mRNA splicing through binding the loop I region of U1-snRNA with its RPM (RNA recognition motif) binding domain. In addition, its RNA binding domain, U1-70K also contains two argine-rich regions, accented with acidic or serine residues, that is thought to have trans-acting functions. Alternative splicing results in 4 known isoforms. Truncated forms of U1-70K are unable to bind RNA.
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Use Anti-U1-70K Antibody, clone 9C4.1 (Mouse Monoclonal Antibody) validated in WB, ICC to detect U1-70K also known as small nuclear ribonucleoprotein 70kDa (U1).
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:500 dilution of a representative lot of this antibody has been shown to detect U1-70K in NIH/3T3, A431, and HeLa cells.
Biological Information
Immunogen
Recombinant protein corresponding to C-terminal domain of human U1-70K.
Epitope
C-terminal domain
Clone
clone 9C4.1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes U1-70K at the C-terminal domain.
FUNCTION: Mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA.
SUBUNIT STRUCTURE: Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B/SFRS10. Interacts with dephosphorylated SFRS13A and SFPQ. Interacts with NUDT21/CPSF5, CPSF6, SFRS2IP, and ZRANB2.
SUBCELLULAR LOCATION: Nucleus.
PTM: The N-terminus is blocked.
Extensively phosphorylated on serine residues in the C-terminal region.
MISCELLANEOUS: Major ribonucleoprotein antigen recognized by the sera from patients with autoimmune diseases, such as systemic lupus erythematosus.
SEQUENCE CAUTION: The sequence CAA28352.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA29964.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA29966.1 differs from that shown. Reason: Erroneous initiation.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected U1-70K on 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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