Ras-related protein Rab-25 (Rab25) is a GTP-binding protein belonging to the Rab family of proteins. Rab25 has observed expression in kidney, lung and gastrointestinal mucosa, appearing to be exclusively expressed within epithelial cells. This specific expression has led to the implication that Rab25 has a role as a regulator for vesicular transport associated with epithelia. Rab25 is potentially a selective regulator for transcytotic pathways and apical recycling. Several recent studies have indicated an observable loss of Rab25 in breast cancer tissue and cell lines, and have suggested a potential role for Rab25 as a regulator for Ras induced tumorigenesis.
Product Information
Format
Purified
Control
MCF-7 cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without 0.05% sodium azide.
Applications
Application
Anti-Rab25 Antibody, clone 12C3 is a Mouse Monoclonal Antibody for the detection of Rab25 & has been validated in WB & IHC.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Imunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Goldenring, J. R. et al. (1996). Am. J. Physiol. 270(33):G515-G525.)
Imunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Casanova, J.E. et al. (1999). Molecular Biology of the Cell. 10:47-61.)
Biological Information
Immunogen
Histidine-tagged recombinant protein corresponding to the C-terminus of rabbit Rab25.
Epitope
C-terminus
Clone
12C3
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes Rab25 at the C-terminus.
Isotype
IgG1κ
Species Reactivity
Human Rabbit Rat Mouse
Species Reactivity Note
Demonstrated to react with human. Predicted to react with mouse, rat, and rabbit based on 100% sequence homology.
FUNCTION: May selectively regulate the apical recycling and/or transcytotic pathways.
SUBUNIT STRUCTURE: Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4 (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
TISSUE SPECIFICTY: Expressed in the gastrointestinal mucosa, (highest expression seen in the ileum and colon), kidney, and lung. A very minor and variable level of expression is seen in the splenic tissue.
SEQUENCE SIMLARITIES: Belongs to the small GTPase superfamily. Rab family.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in MCF-7 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Rab25 on 10 µg of MCF-7 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H, Rb, R, M
WB, IHC
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1574
Description
Anti-Rab25 Antibody, clone 12C3
Alternate Names
RAB25, member RAS oncogene family
ras-related protein Rab-25
Background Information
Ras-related protein Rab-25 (Rab25) is a GTP-binding protein belonging to the Rab family of proteins. Rab25 has observed expression in kidney, lung and gastrointestinal mucosa, appearing to be exclusively expressed within epithelial cells. This specific expression has led to the implication that Rab25 has a role as a regulator for vesicular transport associated with epithelia. Rab25 is potentially a selective regulator for transcytotic pathways and apical recycling. Several recent studies have indicated an observable loss of Rab25 in breast cancer tissue and cell lines, and have suggested a potential role for Rab25 as a regulator for Ras induced tumorigenesis.
Product Information
Format
Purified
Control
MCF-7 cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without 0.05% sodium azide.
Applications
Application
Anti-Rab25 Antibody, clone 12C3 is a Mouse Monoclonal Antibody for the detection of Rab25 & has been validated in WB & IHC.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Imunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Goldenring, J. R. et al. (1996). Am. J. Physiol. 270(33):G515-G525.)
Imunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Casanova, J.E. et al. (1999). Molecular Biology of the Cell. 10:47-61.)
Biological Information
Immunogen
Histidine-tagged recombinant protein corresponding to the C-terminus of rabbit Rab25.
Epitope
C-terminus
Clone
12C3
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes Rab25 at the C-terminus.
Isotype
IgG1κ
Species Reactivity
Human Rabbit Rat Mouse
Species Reactivity Note
Demonstrated to react with human. Predicted to react with mouse, rat, and rabbit based on 100% sequence homology.
FUNCTION: May selectively regulate the apical recycling and/or transcytotic pathways.
SUBUNIT STRUCTURE: Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4 (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
TISSUE SPECIFICTY: Expressed in the gastrointestinal mucosa, (highest expression seen in the ileum and colon), kidney, and lung. A very minor and variable level of expression is seen in the splenic tissue.
SEQUENCE SIMLARITIES: Belongs to the small GTPase superfamily. Rab family.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in MCF-7 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Rab25 on 10 µg of MCF-7 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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