Pax5, also known as B-cell-specific transcription factor (BASP), and encoded by the gene Pax5/Pax-5, plays a role in B cell differentiation and neural development and spermatogenesis. Pax5 regulates the expression of CD19 a B lymphoid specific target gene. Additionally, Pax5 interacts with ETS1 and alters its DNA binding abilities and it interacts with DAXX and TLE4. Pax5 is localized to the nucleus. Pax5 is expressed at early B-cell differentiation and in the developing CNS during development. In the adult it is expressed in the testis. Pax5 is also involved in B-cell related diseases and lymphomas and most recently was shown to be the controlling factor of the “Z” protein BZLF1 which controls the latent to lytic switch of Epstein-Barr virus (EBV) in humans.
Product Information
Format
Purified
Presentation
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Detect PAX5 using this rat monoclonal antibody, Anti-Pax5 Antibody, clone 1H9 validated for use in western blotting & IHC.
Key Applications
Western Blotting
Immunoprecipitation
Immunohistochemistry
Application Notes
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Pax5 in 10 µg of mouse E13-E14 brain tissue lysate.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Isocitrate dehydrogenase in human tonsil and mouse hindbrain tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to mouse Pax5.
Clone
1H9
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Evaluated by Western Blotting in Ramos cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Pax5 in 10 µg of Ramos cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
M, H
WB, IP, IHC
R
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1573
Replaces
MABE517
Description
Anti-Pax5 Antibody, clone 1H9
Alternate Names
Paired box protein Pax-5
BSAP
B-cell-specific transcription factor
Background Information
Pax5, also known as B-cell-specific transcription factor (BASP), and encoded by the gene Pax5/Pax-5, plays a role in B cell differentiation and neural development and spermatogenesis. Pax5 regulates the expression of CD19 a B lymphoid specific target gene. Additionally, Pax5 interacts with ETS1 and alters its DNA binding abilities and it interacts with DAXX and TLE4. Pax5 is localized to the nucleus. Pax5 is expressed at early B-cell differentiation and in the developing CNS during development. In the adult it is expressed in the testis. Pax5 is also involved in B-cell related diseases and lymphomas and most recently was shown to be the controlling factor of the “Z” protein BZLF1 which controls the latent to lytic switch of Epstein-Barr virus (EBV) in humans.
Product Information
Format
Purified
Presentation
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Detect PAX5 using this rat monoclonal antibody, Anti-Pax5 Antibody, clone 1H9 validated for use in western blotting & IHC.
Key Applications
Western Blotting
Immunoprecipitation
Immunohistochemistry
Application Notes
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Pax5 in 10 µg of mouse E13-E14 brain tissue lysate.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Isocitrate dehydrogenase in human tonsil and mouse hindbrain tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to mouse Pax5.
Clone
1H9
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Evaluated by Western Blotting in Ramos cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Pax5 in 10 µg of Ramos cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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