beta-interferon gene positive-regulatory domain I binding factor
PR-domain zinc finger protein 1
Background Information
B-lymphocyte induced maturation protein (BLIMP-1) is a 100 kDa transcriptional repressor containing 1 DET domain and 4 C2H2-type zinc fingers. In the nucleus, BLIMP-1 is known to bind specifically with the Beta-interferon gene positive regulatory domain I (PRDI), and to interact with PRMT5. During B-cell differentiation, BLIMP-1 actively represses the transcription of the gene, c-myc, through binding its promoter and has been deemed a “master regulator” for terminal B-cell differentiation, as it is able to initiate the BCL1 cell developmental cascade.
Product Information
Format
Purified
Control
HL-60 cell lysate
Presentation
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Use Anti-PRDM1 (BLIMP-1) Antibody, clone 6D3 (Rat Monoclonal Antibody) validated in WB, ELISA, IHC, FC to detect PRDM1 (BLIMP-1) also known as PR-domain zinc finger protein 1.
Key Applications
Western Blotting
Flow Cytometry
Immunohistochemistry
ELISA
Application Notes
ELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (Kallies, A., et al. (2004). J Exp Med. 200(8):967-977.)
Flow Cytometry Analysis: A representative lot was used by an independent laboratory in FC. (Kallies, A., et al. (2004). J Exp Med. 200(8):967-977.)
Biological Information
Immunogen
Recombinant protein corresponding to mouse BLIMP-1.
Epitope
Recognizes epitope located between amino acids 255-395 of BLIMP-1
Clone
6D3
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
FUNCTION: Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene. Drives the maturation of B-lymphocytes into Ig secreting cells.
SEQUENCE CAUTION: The sequence AAI29802.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HL-60 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected BLIMP-1 on 10 µg of HL-60 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
beta-interferon gene positive-regulatory domain I binding factor
PR-domain zinc finger protein 1
Background Information
B-lymphocyte induced maturation protein (BLIMP-1) is a 100 kDa transcriptional repressor containing 1 DET domain and 4 C2H2-type zinc fingers. In the nucleus, BLIMP-1 is known to bind specifically with the Beta-interferon gene positive regulatory domain I (PRDI), and to interact with PRMT5. During B-cell differentiation, BLIMP-1 actively represses the transcription of the gene, c-myc, through binding its promoter and has been deemed a “master regulator” for terminal B-cell differentiation, as it is able to initiate the BCL1 cell developmental cascade.
Product Information
Format
Purified
Control
HL-60 cell lysate
Presentation
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Use Anti-PRDM1 (BLIMP-1) Antibody, clone 6D3 (Rat Monoclonal Antibody) validated in WB, ELISA, IHC, FC to detect PRDM1 (BLIMP-1) also known as PR-domain zinc finger protein 1.
Key Applications
Western Blotting
Flow Cytometry
Immunohistochemistry
ELISA
Application Notes
ELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (Kallies, A., et al. (2004). J Exp Med. 200(8):967-977.)
Flow Cytometry Analysis: A representative lot was used by an independent laboratory in FC. (Kallies, A., et al. (2004). J Exp Med. 200(8):967-977.)
Biological Information
Immunogen
Recombinant protein corresponding to mouse BLIMP-1.
Epitope
Recognizes epitope located between amino acids 255-395 of BLIMP-1
Clone
6D3
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
FUNCTION: Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene. Drives the maturation of B-lymphocytes into Ig secreting cells.
SEQUENCE CAUTION: The sequence AAI29802.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HL-60 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected BLIMP-1 on 10 µg of HL-60 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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