p120 Catenin is a cadherin-associated protein that binds intracellular proteins, such as the small GTPase, RhoA, and controls E-cadherin funcation and stability. Proteins of the p120 family and their isoforms are expressed in cells via alternative splicing, and all of them are essential for HGF signaling to Rac1.
Product Information
Format
Purified
Control
Hela cell lysate.
Presentation
Purified mouse monoclonal in 0.1M Tris-Glycine (pH 7.4), 150mM NaCl and 0.05% sodium azide.
Applications
Application
Use Anti-p120 Catenin Antibody, clone 15D2 (mouse monoclonal antibody) validated in WB, ICC, FC to detect p120 Catenin also known as Cadherin-associated Src substrate ,catenin (cadherin-associated protein) delta 1.
Key Applications
Western Blotting
Flow Cytometry
Immunocytochemistry
Application Notes
Immunocytochemistry:
Biological Information
Immunogen
Recombinant fusion protein derived from the C-terminus of murine p120
Epitope
C-terminus
Clone
clone 15D2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Recognizes p120 Catenin
Isotype
IgG1κ
Species Reactivity
Human Rat Mouse
Species Reactivity Note
Human and Mouse. Predicted to react with Rat based on homology.
FUNCTION:Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway By similarity. May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
SUBUNIT STRUCTURE:Belongs to a multiprotein cell-cell adhesion complex that also contains E-cadherin, alpha-catenin, beta-catenin, and gamma-catenin. Binds to the C-terminal fragment of PSEN1 and mutually competes for E-cadherin. Interacts with ZBTB33. Interacts with GLIS2.
SUBCELLULAR LOCATION:Cytoplasm. Nucleus. Cell membraneBy similarity. Note= Interaction with GLIS2 promotes nuclear translocation By similarity.
TISSUE SPECIFICITY:Expressed in vascular endothelium.
INDUCTION:Induced in vascular endothelium by wounding. This effect is potentiated by prior laminar shear stress, which enhances wound closure.
DOMAIN:A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
PTM:Phosphorylated By similarity.
INVOLVEMENT IN DISEASE:May contribute to cell malignancy. Complete loss of expression was observed in approximately 10% of invasive ductal breast carcinomas investigated.
SEQUENCE SIMILARITIES:Belongs to the beta-catenin family.
Contains 10 ARM repeats.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on Hela cell lysate.
Western Blot Analysis: 1 g/mL of this antibody detected p120 Catenin in lysate from Hela cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H, R, M
WB, FC, ICC
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1567
Replaces
04-237
Description
Anti-p120 Catenin Antibody, clone 15D2
Alternate Names
Cadherin-associated Src substrate
catenin, delta 1, p120 catenin
catenin (cadherin-associated protein), delta 1
Background Information
p120 Catenin is a cadherin-associated protein that binds intracellular proteins, such as the small GTPase, RhoA, and controls E-cadherin funcation and stability. Proteins of the p120 family and their isoforms are expressed in cells via alternative splicing, and all of them are essential for HGF signaling to Rac1.
Product Information
Format
Purified
Control
Hela cell lysate.
Presentation
Purified mouse monoclonal in 0.1M Tris-Glycine (pH 7.4), 150mM NaCl and 0.05% sodium azide.
Applications
Application
Use Anti-p120 Catenin Antibody, clone 15D2 (mouse monoclonal antibody) validated in WB, ICC, FC to detect p120 Catenin also known as Cadherin-associated Src substrate ,catenin (cadherin-associated protein) delta 1.
Key Applications
Western Blotting
Flow Cytometry
Immunocytochemistry
Application Notes
Immunocytochemistry:
Biological Information
Immunogen
Recombinant fusion protein derived from the C-terminus of murine p120
Epitope
C-terminus
Clone
clone 15D2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Recognizes p120 Catenin
Isotype
IgG1κ
Species Reactivity
Human Rat Mouse
Species Reactivity Note
Human and Mouse. Predicted to react with Rat based on homology.
FUNCTION:Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway By similarity. May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
SUBUNIT STRUCTURE:Belongs to a multiprotein cell-cell adhesion complex that also contains E-cadherin, alpha-catenin, beta-catenin, and gamma-catenin. Binds to the C-terminal fragment of PSEN1 and mutually competes for E-cadherin. Interacts with ZBTB33. Interacts with GLIS2.
SUBCELLULAR LOCATION:Cytoplasm. Nucleus. Cell membraneBy similarity. Note= Interaction with GLIS2 promotes nuclear translocation By similarity.
TISSUE SPECIFICITY:Expressed in vascular endothelium.
INDUCTION:Induced in vascular endothelium by wounding. This effect is potentiated by prior laminar shear stress, which enhances wound closure.
DOMAIN:A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
PTM:Phosphorylated By similarity.
INVOLVEMENT IN DISEASE:May contribute to cell malignancy. Complete loss of expression was observed in approximately 10% of invasive ductal breast carcinomas investigated.
SEQUENCE SIMILARITIES:Belongs to the beta-catenin family.
Contains 10 ARM repeats.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot on Hela cell lysate.
Western Blot Analysis: 1 g/mL of this antibody detected p120 Catenin in lysate from Hela cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
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