The hnRNP C proteins (C1/C2) are nuclear pre-mRNA-binding proteins that belong to the large RNP motif family of RNA-binding proteins. The proteins encoded by this gene act as a tetramer and are involved in the assembly of 40S hnRNP particles. The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins aassociate with pre-mRNAs in the nucleus and influence pre-mRNA processing and other aspects of mRNA metabolism and transport. In vivo, hnRNP C proteins have been found to interact with Grb2.
Product Information
Format
Purified
Control
HeLa nuclear extract lysate
Presentation
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide
Applications
Application
Anti-HnRNP C1/C2 Antibody, clone 4F4 is a Mouse Monoclonal Antibody for detection of HnRNP C1/C2 also known as heterogeneous nuclear ribonucleoprotein C (C1/C2) & has been validated in WB.
Key Applications
Western Blotting
Biological Information
Immunogen
RNP’s eluted from oligo (dT) cellulose column of human HnRNP C1/C2.
Epitope
Unknown
Clone
4F4
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes HnRNP C1/C2.
Isotype
IgG2bκ
Species Reactivity
Human Mouse
Species Reactivity Note
Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene. [provided by RefSeq].
FUNCTION: Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules.
SUBUNIT STRUCTURE: Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. Assembly of 3 tetramers with bound pre-mRNA gives rise to a 19S complex that interacts with HNRNPA2B1 tetramers. Component of the 40S hnRNP particle. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRNPA1, HNRNPA2B1, HNRPA3, HNRNPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRNPR, HNRNPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Interacts with IGF2BP1.
SUBCELLULAR LOCATION: Nucleus. Note: Component of ribonucleosomes.
PTM: Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
Sumoylated. Sumoylation reduces affinity for mRNA.
SEQUENCE SIMILARITIES: Belongs to the RRM HNRPC family. RALY subfamily.
Contains 1 RRM (RNA recognition motif) domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa nuclear extract lysate.
Western Blot Analysis: 0.01 µg/ml of this antibody detected HnRNP C1/C2 on 10 µg of HeLa nuclear extract lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H, M
WB
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1520
Description
Anti-HnRNP C1/C2 Antibody, clone 4F4
Alternate Names
heterogeneous nuclear ribonucleoprotein C
nuclear ribonucleoprotein particle C2 protein
nuclear ribonucleoprotein particle C1 protein
hnRNP C1 / hnRNP C2
heterogeneous nuclear ribonucleoprotein C (C1/C2)
Background Information
The hnRNP C proteins (C1/C2) are nuclear pre-mRNA-binding proteins that belong to the large RNP motif family of RNA-binding proteins. The proteins encoded by this gene act as a tetramer and are involved in the assembly of 40S hnRNP particles. The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins aassociate with pre-mRNAs in the nucleus and influence pre-mRNA processing and other aspects of mRNA metabolism and transport. In vivo, hnRNP C proteins have been found to interact with Grb2.
Product Information
Format
Purified
Control
HeLa nuclear extract lysate
Presentation
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide
Applications
Application
Anti-HnRNP C1/C2 Antibody, clone 4F4 is a Mouse Monoclonal Antibody for detection of HnRNP C1/C2 also known as heterogeneous nuclear ribonucleoprotein C (C1/C2) & has been validated in WB.
Key Applications
Western Blotting
Biological Information
Immunogen
RNP’s eluted from oligo (dT) cellulose column of human HnRNP C1/C2.
Epitope
Unknown
Clone
4F4
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes HnRNP C1/C2.
Isotype
IgG2bκ
Species Reactivity
Human Mouse
Species Reactivity Note
Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene. [provided by RefSeq].
FUNCTION: Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules.
SUBUNIT STRUCTURE: Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. Assembly of 3 tetramers with bound pre-mRNA gives rise to a 19S complex that interacts with HNRNPA2B1 tetramers. Component of the 40S hnRNP particle. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRNPA1, HNRNPA2B1, HNRPA3, HNRNPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRNPR, HNRNPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Interacts with IGF2BP1.
SUBCELLULAR LOCATION: Nucleus. Note: Component of ribonucleosomes.
PTM: Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
Sumoylated. Sumoylation reduces affinity for mRNA.
SEQUENCE SIMILARITIES: Belongs to the RRM HNRPC family. RALY subfamily.
Contains 1 RRM (RNA recognition motif) domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa nuclear extract lysate.
Western Blot Analysis: 0.01 µg/ml of this antibody detected HnRNP C1/C2 on 10 µg of HeLa nuclear extract lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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