Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as Erk1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator of the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs), GPCR, and/or integrin stimulation. This activates the Ras-Raf-MEK-Erk pathway that results in the phosphorylation/activation of Erk1/2 (p44/p42) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 & Erk2, respectively).
Product Information
Format
Purified
Control
HeLa cell lysate. For western blots load 5-10 µg Rat Pituitary Lysate.
Presentation
Purified mouse monoclonal IgG1 in aqueous buffered solution containing 50% glycerol, BSA and ≤0.09% sodium azide.
Applications
Application
Detect Erk1/2 (p44/p42) using this Anti-Erk1/2 (p44/p42) Antibody, clone MK12 validated for use in WB, IP, IH(P) & IC.
Key Applications
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Application Notes
Immunocytochemistry: A previous lot of this antibody was used in IC.
Immunoprecipitation: A previous lot of this antibody was used in IP.
Optimal working dilutions must be determined by end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of ERK1/2 with Citrate pH 6.0 Epitope Retrieval: Human Placenta
.Erk 1/2 representative staining pattern/morphology in human placenta. Tissue pretreated with citrate pH 6, antigen retrieval. A previous lot of
this antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB.
Biological Information
Immunogen
Synthetic peptide corresponding to positions 325-345 of rat ERK1.
Clone
MK12
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
ERK1 is the 44 kDa member of the ERK family and shares 85% homology with ERK2. By Western blot, this monoclonal recognizes 44 kDa and 42 kDa proteins from multiple cell lysates including NIH/3T3, RAW264, C2C12, and PC12 as well as tissue samples such as rat pituitary.
Isotype
IgG1
Species Reactivity
Human Chicken Bovine Xenopus Canine Rat Mouse
Species Reactivity Note
Human, Mouse, Rat, Canine, Xenopus, Bovine, and Chicken.
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq]
FUNCTION: Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium (By similarity).
ENZYME REGULATION: Activated by tyrosine phosphorylation in response to insulin and NGF.
SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef. This interaction inhibits its kinase activity. Interacts with HSF4 and NISCH.
DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
SIMILARITY: Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by Western Blot on HeLa lysates.
Western Blot Analysis: 1:5,000 dilution of this lot detected ERK1/2 (MAPK) in NIH/3T3 cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H, Ch, B, Xn, Ca, R, M
WB, ICC, IH(P), IP
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1152
Replaces
MAB3054
Description
Anti-Erk1/2 (p44/p42) Antibody, clone MK12
Alternate Names
Mitogen-activated protein kinase
Extracellular signal-regulated kinase
Background Information
Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as Erk1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator of the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs), GPCR, and/or integrin stimulation. This activates the Ras-Raf-MEK-Erk pathway that results in the phosphorylation/activation of Erk1/2 (p44/p42) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 & Erk2, respectively).
Product Information
Format
Purified
Control
HeLa cell lysate. For western blots load 5-10 µg Rat Pituitary Lysate.
Presentation
Purified mouse monoclonal IgG1 in aqueous buffered solution containing 50% glycerol, BSA and ≤0.09% sodium azide.
Applications
Application
Detect Erk1/2 (p44/p42) using this Anti-Erk1/2 (p44/p42) Antibody, clone MK12 validated for use in WB, IP, IH(P) & IC.
Key Applications
Western Blotting
Immunocytochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Application Notes
Immunocytochemistry: A previous lot of this antibody was used in IC.
Immunoprecipitation: A previous lot of this antibody was used in IP.
Optimal working dilutions must be determined by end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of ERK1/2 with Citrate pH 6.0 Epitope Retrieval: Human Placenta
.Erk 1/2 representative staining pattern/morphology in human placenta. Tissue pretreated with citrate pH 6, antigen retrieval. A previous lot of
this antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB.
Biological Information
Immunogen
Synthetic peptide corresponding to positions 325-345 of rat ERK1.
Clone
MK12
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
ERK1 is the 44 kDa member of the ERK family and shares 85% homology with ERK2. By Western blot, this monoclonal recognizes 44 kDa and 42 kDa proteins from multiple cell lysates including NIH/3T3, RAW264, C2C12, and PC12 as well as tissue samples such as rat pituitary.
Isotype
IgG1
Species Reactivity
Human Chicken Bovine Xenopus Canine Rat Mouse
Species Reactivity Note
Human, Mouse, Rat, Canine, Xenopus, Bovine, and Chicken.
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq]
FUNCTION: Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium (By similarity).
ENZYME REGULATION: Activated by tyrosine phosphorylation in response to insulin and NGF.
SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef. This interaction inhibits its kinase activity. Interacts with HSF4 and NISCH.
DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
SIMILARITY: Contains 1 protein kinase domain.
Product Usage Statements
Quality Assurance
Routinely evaluated by Western Blot on HeLa lysates.
Western Blot Analysis: 1:5,000 dilution of this lot detected ERK1/2 (MAPK) in NIH/3T3 cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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