The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (>95%).
Product Information
Format
Purified
Control
Included Positive Control: Cat.# 12-628, Calyculin A/Okadaic-treated A431 cell lysate is provided as a free positive control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20ºC. The lysate is stable for 6 months at -20ºC.
Presentation
Purified mouse monoclonal IgG1 in buffer containing PBS with 0.1% sodium azide and 30% glycerol.
Applications
Application
Detect Phosphoserine using this Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1) validated for use in ELISA, FC, IF, IH(P) & WB.
Key Applications
ELISA
Western Blotting
Immunohistochemistry (Paraffin)
Immunofluorescence
Flow Cytometry
Application Notes
Immunofluorescence
Flow Cytometry
Immunohistochemistry (Paraffin)
ELISA
Biological Information
Immunogen
Phosphoserine coupled to KLH.
Clone
4A4
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes serine-phosphorylated proteins from all species.
Isotype
IgG1
Species Reactivity
Vertebrates
Species Reactivity Note
Reacts with vertebrates
Antibody Type
Monoclonal Antibody
Modifications
Phosphorylation
Purification Method
Protein G-Sepharose™ chromatography
Product Usage Statements
Quality Assurance
Routinely evaluated by Western Blot analysis on lysate from Calyculin A/Okadaic-treated human A431 carcinoma cells.
Western Blot Analysis:
0.5–2 μg/mL of this lot detected serinephosphorylated proteins in a lysate from either insulin or Calyculin A/Okadaic-treated human A431 carcinoma cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
For maximum recovery, centrifuge the original vial prior to cap removal. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.
The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (>95%).
Product Information
Format
Purified
Control
Included Positive Control: Cat.# 12-628, Calyculin A/Okadaic-treated A431 cell lysate is provided as a free positive control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20ºC. The lysate is stable for 6 months at -20ºC.
Presentation
Purified mouse monoclonal IgG1 in buffer containing PBS with 0.1% sodium azide and 30% glycerol.
Applications
Application
Detect Phosphoserine using this Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1) validated for use in ELISA, FC, IF, IH(P) & WB.
Key Applications
ELISA
Western Blotting
Immunohistochemistry (Paraffin)
Immunofluorescence
Flow Cytometry
Application Notes
Immunofluorescence
Flow Cytometry
Immunohistochemistry (Paraffin)
ELISA
Biological Information
Immunogen
Phosphoserine coupled to KLH.
Clone
4A4
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes serine-phosphorylated proteins from all species.
Isotype
IgG1
Species Reactivity
Vertebrates
Species Reactivity Note
Reacts with vertebrates
Antibody Type
Monoclonal Antibody
Modifications
Phosphorylation
Purification Method
Protein G-Sepharose™ chromatography
Product Usage Statements
Quality Assurance
Routinely evaluated by Western Blot analysis on lysate from Calyculin A/Okadaic-treated human A431 carcinoma cells.
Western Blot Analysis:
0.5–2 μg/mL of this lot detected serinephosphorylated proteins in a lysate from either insulin or Calyculin A/Okadaic-treated human A431 carcinoma cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20ºC from date of receipt.
For maximum recovery, centrifuge the original vial prior to cap removal. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.
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