secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
secreted phosphoprotein 1
osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein
bone sialoprotein I
Urinary stone protein
SPP1/CALPHA1 fusion
Background Information
Osteopontin is a single-chain polypeptide. It is highly phosphorylated sialoprotein that is a prominent component of the mineralized extracellular matrices of bones and teeth. It is characterized by the presence of a polyaspartic acid sequence and sites of Ser/Thr phosphorylation that mediate hydroxyapatite binding, and a highly conserved RGD motif that mediates cell attachment/signaling. Expression of osteopontin in a variety of tissues indicates a multiplicity of functions that involve one or more of these conserved motifs. While the lack of a clear phenotype in osteopontin "knockout" mice has not established a definitive role for osteopontin in any tissue, recent studies have provided some novel and intriguing insights into the versatility of this enigmatic protein in diverse biological events, including developmental processes, wound healing, immunological responses, tumorigenesis, bone resorption, and calcification. The ability of osteopontin to stimulate cell activity through multiple receptors linked to several interactive signaling pathways can account for much of the functional diversity.
Product Information
Format
Purified
Control
Human serum lysate
Presentation
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Applications
Application
Anti-Osteopontin Antibody, clone 6B6.2 detects level of Osteopontin & has been published & validated for use in WB.
Key Applications
Western Blotting
Application Notes
Western Blot Analysis: 0.5 µg/mL from a representative lot detected Osteopontin on 10 µg of mouse kidney lysate.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of human Osteopontin.
Epitope
C-terminus
Clone
6B6.2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes Osteopontin at the C-terminus.
FUNCTION: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity).
SUBUNIT STRUCTURE: Ligand for integrin alpha-V/beta-3.
SUBCELLULAR LOCATION: Secreted.
PTM: Extensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
DISEASE: This protein plays a principal role in urinary stone formation as the stone matrix.
SEQUENCE SIMILARITY: Belongs to the osteopontin family.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in human serum lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Osteopontin on 10 µg of human serum lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H, M
WB
M
Purified
Monoclonal Antibody
Description
Catalogue Number
04-970
Description
Anti-Osteopontin Antibody, clone 6B6.2
Alternate Names
Bone sialoprotein 1
osteopontin
Uropontin
secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
secreted phosphoprotein 1
osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein
bone sialoprotein I
Urinary stone protein
SPP1/CALPHA1 fusion
Background Information
Osteopontin is a single-chain polypeptide. It is highly phosphorylated sialoprotein that is a prominent component of the mineralized extracellular matrices of bones and teeth. It is characterized by the presence of a polyaspartic acid sequence and sites of Ser/Thr phosphorylation that mediate hydroxyapatite binding, and a highly conserved RGD motif that mediates cell attachment/signaling. Expression of osteopontin in a variety of tissues indicates a multiplicity of functions that involve one or more of these conserved motifs. While the lack of a clear phenotype in osteopontin "knockout" mice has not established a definitive role for osteopontin in any tissue, recent studies have provided some novel and intriguing insights into the versatility of this enigmatic protein in diverse biological events, including developmental processes, wound healing, immunological responses, tumorigenesis, bone resorption, and calcification. The ability of osteopontin to stimulate cell activity through multiple receptors linked to several interactive signaling pathways can account for much of the functional diversity.
Product Information
Format
Purified
Control
Human serum lysate
Presentation
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Applications
Application
Anti-Osteopontin Antibody, clone 6B6.2 detects level of Osteopontin & has been published & validated for use in WB.
Key Applications
Western Blotting
Application Notes
Western Blot Analysis: 0.5 µg/mL from a representative lot detected Osteopontin on 10 µg of mouse kidney lysate.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of human Osteopontin.
Epitope
C-terminus
Clone
6B6.2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes Osteopontin at the C-terminus.
FUNCTION: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity).
SUBUNIT STRUCTURE: Ligand for integrin alpha-V/beta-3.
SUBCELLULAR LOCATION: Secreted.
PTM: Extensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
DISEASE: This protein plays a principal role in urinary stone formation as the stone matrix.
SEQUENCE SIMILARITY: Belongs to the osteopontin family.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in human serum lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Osteopontin on 10 µg of human serum lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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