Rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Applications
Application
Anti-phospho-MSK1 (S376) Antibody is an antibody against phospho-MSK1 (S376) for use in FC, IH(P), IP & WB.
Key Applications
Western Blotting
Flow Cytometry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Application Notes
Western Blot: 1:5000.
Immunoprecipitation: 1:30.
Immunofluorescence: 1:100-1:250.
Immunohistochemistry: 1:50-1:100 on paraffin-embedded sections of human urinary bladder carcinoma.
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
KLH-conjugated synthetic phospho-peptide corresponding to residues surrounding Serine 376 of human MSK1.
FUNCTION:SwissProt: O75582 # Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14). COFACTOR:Magnesium. SIZE:802 amino acids; 89865 Da SUBUNIT:Forms a complex with either ERK1 or ERK2 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA5 associates with and phosphorylates the NF-kappa-B p65 subunit RELA. SUBCELLULAR LOCATION:Nucleus. Cytoplasm. Note=Predominantly nuclear. Partially cytoplasmic. TISSUE SPECIFICITY:Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver. PTM:Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C- terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. SIMILARITY:SwissProt: O75582 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 2 protein kinase domains. MISCELLANEOUS:Enzyme activity requires the presence of both kinase domains.
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot analysis.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Applications
Application
Anti-phospho-MSK1 (S376) Antibody is an antibody against phospho-MSK1 (S376) for use in FC, IH(P), IP & WB.
Key Applications
Western Blotting
Flow Cytometry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Application Notes
Western Blot: 1:5000.
Immunoprecipitation: 1:30.
Immunofluorescence: 1:100-1:250.
Immunohistochemistry: 1:50-1:100 on paraffin-embedded sections of human urinary bladder carcinoma.
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
KLH-conjugated synthetic phospho-peptide corresponding to residues surrounding Serine 376 of human MSK1.
FUNCTION:SwissProt: O75582 # Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14). COFACTOR:Magnesium. SIZE:802 amino acids; 89865 Da SUBUNIT:Forms a complex with either ERK1 or ERK2 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA5 associates with and phosphorylates the NF-kappa-B p65 subunit RELA. SUBCELLULAR LOCATION:Nucleus. Cytoplasm. Note=Predominantly nuclear. Partially cytoplasmic. TISSUE SPECIFICITY:Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver. PTM:Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C- terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. SIMILARITY:SwissProt: O75582 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 2 protein kinase domains. MISCELLANEOUS:Enzyme activity requires the presence of both kinase domains.
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot analysis.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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